Kiguchi Kazushige, Ishiwata Isamu, Ishiwata Emiko, Koshitaka Yutaka, Ohara Tatsuru, Okudai Yoshiko, Sato Sojiro, Kobayashi Youichi, Ishizuka Bunpei, Ishikawa Hiroshi
Department of Obstetrics & Gynecology, St. Marianna University.
Hum Cell. 2005 Mar;18(1):45-52. doi: 10.1111/j.1749-0774.2005.tb00056.x.
A cell line designated HTLS was established from the retroperitoneal liposarcoma. The HTLS line showed stable proliferation without interruption for 2 years and subcultivated over 35 times. The cells were elongated fibrous and spindle in shape, and neoplastic and pleomorphic features. The multinucleated giant cells with fine cytoplasm were seen. The cells proliferated slowly and the population doubling time was about 90 hours. The chromosome number showed a wide distribution of aneuploidy, the mode was hyperdiploid range (51-52), and many marker chromosomes were observed. The cells were transplantable into the submucosa of immunesuppressed hamster's cheek pouch and produced liposarcoma, while were not transplantable into subcutis of nude mice
一种名为HTLS的细胞系是从腹膜后脂肪肉瘤中建立的。HTLS细胞系在2年内持续稳定增殖且未中断，传代超过35次。细胞呈细长纤维状和纺锤形，具有肿瘤性和多形性特征。可见具有细细胞质的多核巨细胞。细胞增殖缓慢，群体倍增时间约为90小时。染色体数目显示非整倍体分布广泛，众数在超二倍体范围（51 - 52），并观察到许多标记染色体。这些细胞可移植到免疫抑制仓鼠颊囊的黏膜下层并产生脂肪肉瘤，但不能移植到裸鼠的皮下。
