Richardson M A, Berg D T, Johnston P A, McClure D, Grinnell B W
Cardiovascular Research Division, Lilly Corporate Center, Indianapolis, IN 46285-0522, USA.
J Lipid Res. 1996 May;37(5):1162-6.
Cholesteryl ester transfer protein (CETP) mediates the exchange of phospholipids and neutral lipids between the plasma lipoproteins, and plays an important role in high density lipoprotein (HDL) metabolism. While there are reports of low-level CETP secretion from cultured cells, the lack of a good model cell line has hampered the detailed study of CETP regulation and secretion. In this study, we have found that the human liposarcoma cell line, SW872, secretes cholesteryl ester transfer protein at levels substantially higher than observed from other cell lines. The secretion of CETP from this adipose-derived cell was up-regulated by 25-OH cholesterol and by low density lipoprotein (LDL) cholesterol in a concentration-dependent manner. Analysis of both full length and exon 9-deleted CETP mRNA demonstrated increases in response to LDL and 25-OH cholesterol, providing evidence for regulation at the message level. Our results suggest that the CETP-producing SW872 cell line may provide a model in which to study the regulation of this important modulator of lipoprotein metabolism.
胆固醇酯转运蛋白（CETP）介导血浆脂蛋白之间磷脂和中性脂质的交换，在高密度脂蛋白（HDL）代谢中起重要作用。虽然有报道称培养细胞分泌的CETP水平较低，但缺乏良好的模型细胞系阻碍了对CETP调节和分泌的详细研究。在本研究中，我们发现人脂肪肉瘤细胞系SW872分泌胆固醇酯转运蛋白的水平显著高于其他细胞系。这种脂肪来源细胞分泌的CETP被25-羟基胆固醇和低密度脂蛋白（LDL）胆固醇以浓度依赖的方式上调。对全长和缺失外显子9的CETP mRNA的分析表明，其响应LDL和25-羟基胆固醇而增加，这为在信息水平上的调节提供了证据。我们的结果表明，产生CETP的SW872细胞系可能提供一个模型，用于研究这种脂蛋白代谢重要调节因子的调节机制。
