Zhu Y-B, Li H-Y, Yang F
Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, Xiamen, PR China.
Arch Virol. 2006 Jan;151(1):71-82. doi: 10.1007/s00705-005-0612-z. Epub 2005 Aug 23.
White spot syndrome virus (WSSV) was purified from the tissues of experimentally infected crayfish (Procambarus clarkii) with high yield. Based on SDS-PAGE of purified WSSV and mass spectrometry analysis, a protein with the molecular mass of 39 kDa was identified to match an open reading frame (ORF), WSV339, of WSSV genome. This ORF was 849 bp in length, encoding a 283 amino acid polypeptide. The protein was named VP39 and its gene was termed as vp39. Temporal transcription analysis revealed that vp39 was a late gene. The gene was cloned into pET-GST vector and expressed as a fusion protein with glutathione S-transferase (GST) in Escherichia coli strain BL21 (DE3). Western blot analysis indicated that VP39 could be detected in the extracts of both the WSSV virions and the viral envelopes. It was further located in the WSSV virions as an envelope protein using immunoelectron microscopy.
白斑综合征病毒（WSSV）从实验感染的小龙虾（克氏原螯虾）组织中高产纯化。基于纯化的WSSV的SDS-PAGE和质谱分析，鉴定出一种分子量为39 kDa的蛋白质，与WSSV基因组的一个开放阅读框（ORF）WSV339匹配。该ORF长度为849 bp，编码一个283个氨基酸的多肽。该蛋白质被命名为VP39，其基因被称为vp39。时序转录分析表明vp39是一个晚期基因。该基因被克隆到pET-GST载体中，并在大肠杆菌BL21（DE3）菌株中作为与谷胱甘肽S-转移酶（GST）的融合蛋白表达。蛋白质印迹分析表明，在WSSV病毒粒子和病毒包膜的提取物中均能检测到VP39。使用免疫电子显微镜进一步将其定位为WSSV病毒粒子中的一种包膜蛋白。
