Chen Jing, Li Zhengjun, Hew Choy-Leong
Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, 117543, Singapore.
Virology. 2007 Jul 20;364(1):208-13. doi: 10.1016/j.virol.2007.02.030. Epub 2007 Apr 2.
White spot syndrome virus is one of the most serious viral pathogens causing huge mortality in shrimp farming. Here we report characterization of WSV010, a novel structural protein identified by our recent shotgun proteomics study. Its ORF contains 294 nucleotides encoding 97 amino acids. Transcription analysis using RT-PCR showed that wsv010 is a late gene. Localization analyses by Western blot and immunoelectron microscopy demonstrated that WSV010 is a viral envelope protein. Furthermore, the pull-down assay revealed that WSV010 could interact with VP24, which is a major envelope protein. Since WSV010 lacks a transmembrane domain, these results suggest that WSV010 may anchor to the envelope through interaction with VP24. Previous studies indicated that VP24 could also interact with VP28 and VP26. Therefore, we propose that VP24 may act as a linker protein to associate these envelope proteins together to form a complex, which may play an important role in viral morphogenesis and viral infection.
白斑综合征病毒是导致对虾养殖中大量死亡的最严重病毒病原体之一。在此，我们报告了WSV010的特性，它是我们最近通过鸟枪法蛋白质组学研究鉴定出的一种新型结构蛋白。其开放阅读框包含294个核苷酸，编码97个氨基酸。使用逆转录聚合酶链反应（RT-PCR）进行的转录分析表明，wsv010是一个晚期基因。通过蛋白质免疫印迹法和免疫电子显微镜进行的定位分析表明，WSV010是一种病毒包膜蛋白。此外，下拉实验表明WSV010可与主要包膜蛋白VP24相互作用。由于WSV010缺乏跨膜结构域，这些结果表明WSV010可能通过与VP24相互作用而锚定在包膜上。先前的研究表明，VP24也可与VP28和VP26相互作用。因此，我们提出VP24可能作为连接蛋白将这些包膜蛋白结合在一起形成一个复合体，这可能在病毒形态发生和病毒感染中起重要作用。
