Fregel Rosa, Maca-Meyer Nicole, Cabrera Vicente Martínez, González Ana María, Larruga José María
Departamento de Genética, Facultad de BiologíaFacultad de Biología, Universidad de La Laguna, Tenerife, Spain.
Immunogenetics. 2005 Sep;57(8):572-8. doi: 10.1007/s00251-005-0029-6. Epub 2005 Sep 29.
A simple and affordable multiplex polymerase chain reaction-single-strand conformation polymorphism method is proposed for the molecular study of AB0 polymorphisms. Application of this method to the peopling of the Canary Islands, analyzing a total of 2,200 chromosomes, detected that in addition to Berbers and Basques, the rare alleles 0210 and O303 are also present in the Iberian Peninsula and in the Canary Islands. Allele B101, with the highest frequency in Northwest (NW) Africa, shows a negative correlation (R = -0.822, p = 0.023) between geographic distances from this continent and insular frequencies, congruent with a main aborigine colonization from East to West still detectable today. Similar to previous autosomal studies, admixture estimations point to a major Iberian contribution (82 +/- 0.5%) to the Canary Islands, although, in some islands as La Gomera, the NW African component raised to 62 +/- 4.3%.
本文提出了一种简单且经济实惠的多重聚合酶链反应-单链构象多态性方法，用于AB0多态性的分子研究。将该方法应用于加那利群岛的人口研究，共分析了2200条染色体，结果发现，除了柏柏尔人和巴斯克人外，罕见等位基因0210和O303在伊比利亚半岛和加那利群岛也有出现。在西北非洲频率最高的等位基因B101，在与该大陆的地理距离和岛屿频率之间呈现负相关（R = -0.822，p = 0.023），这与至今仍可检测到的从东到西的主要原住民殖民情况相符。与之前的常染色体研究相似，混合估计表明伊比利亚人对加那利群岛的贡献最大（82±0.5%），不过，在一些岛屿如拉戈梅拉岛，西北非洲成分上升至62±4.3%。
