Deveau Jason S T, Lindinger Michael I, Grodzinski Bernard
Department of Plant Agriculture, University of Guelph, Guelph, Ontario, N1G 2W1, Canada.
Biol Proced Online. 2005;7:31-40. doi: 10.1251/bpo103. Epub 2005 Apr 18.
We describe an improved, efficient and reliable method for the vapour-phase silanization of multi-barreled, ion-selective microelectrodes of which the silanized barrel(s) are to be filled with neutral liquid ion-exchanger (LIX). The technique employs a metal manifold to exclusively and simultaneously deliver dimethyldichlorosilane to only the ion-selective barrels of several multi-barreled microelectrodes. Compared to previously published methods the technique requires fewer procedural steps, less handling of individual microelectrodes, improved reproducibility of silanization of the selected microelectrode barrels and employs standard borosilicate tubing rather than the less-conventional theta-type glass. The electrodes remain stable for up to 3 weeks after the silanization procedure. The efficacy of a double-barreled electrode containing a proton ionophore in the ion-selective barrel is demonstrated in situ in the leaf apoplasm of pea (Pisum) and sunflower (Helianthus). Individual leaves were penetrated to depth of approximately 150 microm through the abaxial surface. Microelectrode readings remained stable after multiple impalements without the need for a stabilizing PVC matrix.
我们描述了一种改进的、高效且可靠的方法，用于对多管离子选择性微电极进行气相硅烷化处理，其中经硅烷化处理的管要填充中性液体离子交换剂（LIX）。该技术采用金属歧管专门且同时仅将二甲基二氯硅烷输送到多个多管微电极的离子选择性管中。与先前发表的方法相比，该技术所需的操作步骤更少，对单个微电极的处理更少，所选微电极管硅烷化的重现性更高，并且使用标准硼硅酸盐管材而非不太常见的θ型玻璃。硅烷化处理后，电极可保持稳定长达3周。在豌豆（Pisum）和向日葵（Helianthus）叶片质外体中原位证明了离子选择性管中含有质子离子载体的双管电极的功效。通过叶片背面将单个叶片穿透至约150微米的深度。多次穿刺后微电极读数保持稳定，无需稳定的PVC基质。
