Borrelli M J, Carlini W G, Dewey W C, Ransom B R
J Neurosci Methods. 1985 Oct-Nov;15(2):141-54. doi: 10.1016/0165-0270(85)90051-2.
A simple procedure for manufacturing Cl-, K+, and pH liquid membrane ion-sensitive microelectrodes is presented in detail. Electrodes suitable for recording from the specimen of interest are back-filled with a small amount of silane solution and heated for 5 min on a hot plate at a temperature between 400 and 500 degrees C, after which they are injected with the ion-sensitive resin. The procedure is adaptable to many different glass stocks, e.g., single-barreled, double-barreled, or theta glass, and can be used to produce electrodes having a wide range of tip sizes for recording either extracellular or intracellular ion activities. Another advantage of the method is speed; up to 10 electrodes can be prepared simultaneously, permitting over 40 functional electrodes to be made per hour.
本文详细介绍了一种制造氯离子、钾离子和pH值液体膜离子敏感微电极的简单方法。适合于从感兴趣的标本进行记录的电极，先反向填充少量硅烷溶液，然后在400至500摄氏度的热板上加热5分钟，之后注入离子敏感树脂。该方法适用于许多不同的玻璃管，例如单管、双管或θ形玻璃管，并且可用于生产具有多种尖端尺寸的电极，以记录细胞外或细胞内的离子活性。该方法的另一个优点是速度快；可同时制备多达10个电极，每小时可制作40多个功能电极。
