Zerwekh J E, Kourosh S, Scheinberg R, Kitano T, Edwards M L, Shin D, Selby D K
Department of Orthopaedic Surgery, University of Texas Southwestern Medical Center, Dallas 75235-8885.
J Orthop Res. 1992 Jul;10(4):562-72. doi: 10.1002/jor.1100100411.
The osteoconductive capacity of fibrillar collagen-biphasic calcium phosphate composition was compared to autogenous bone in a canine spinal fusion model. All animals underwent a standard intervertebral body fusion (L2-L4) with rigid internal fixation and received either autogenous bone alone or a mixture of the ceramic and autogenous bone (3:1) as the graft material. Animals were followed for 12 months and the quality of fusion in each animal assessed by biomechanical testing and histological analysis. The fused L2-L4 segment of each dog was embedded in bone cement and mounted in a specially designed mechanical tester for testing in flexion, extension, and side bending. Overall, the mean rigidity of the fusion mass was not significantly different between the two groups [10.5 +/- 4.1 (SD) for autogenous bone vs. 11.3 +/- 1.7 for the ceramic plus autogenous bone, p greater than 0.05]. Similar findings were obtained for mean bending moment, compressive load, angular deformation, and energy absorbed for the two groups. Histological analysis was performed on transverse nondecalcified specimens. Quantitation of bone ingrowth using back-scattered electron imaging disclosed no significant differences in the amount of new bone formed at the graft site between autogenous bone and the ceramic plus autogenous bone recipients (23.4 +/- 10% vs. 25.8 +/- 8.8%) when correction for the autogenous bone volumes was performed. Light microscopic analysis of toluidine blue-stained transverse sections demonstrated new bone growth around and through the ceramic bone graft material. These results suggest that use of a collagen-biphasic calcium phosphate ceramic and autogenous bone mixture (3:1) provides a suitable osteoconductive alternative to the use of autogenous bone and results in the formation of a mechanically competent fusion mass not significantly different from that obtained with autogenous bone alone.
在犬类脊柱融合模型中，将纤维状胶原 - 双相磷酸钙组合物的骨传导能力与自体骨进行了比较。所有动物均接受了标准的椎体间融合术（L2 - L4）并进行了坚固的内固定，且分别接受了单独的自体骨或陶瓷与自体骨的混合物（3:1）作为移植材料。对动物进行了12个月的随访，并通过生物力学测试和组织学分析评估每只动物的融合质量。将每只狗融合的L2 - L4节段嵌入骨水泥中，并安装在专门设计的机械测试仪中进行屈曲、伸展和侧弯测试。总体而言，两组之间融合块的平均刚度无显著差异[自体骨组为10.5±4.1（标准差），陶瓷加自体骨组为11.3±1.7，p大于0.05]。两组在平均弯矩、压缩载荷、角变形和吸收能量方面也得到了类似的结果。对横向未脱钙标本进行了组织学分析。使用背散射电子成像对骨长入进行定量分析，结果显示，在对自体骨体积进行校正后，自体骨组和陶瓷加自体骨组在移植部位形成的新骨量无显著差异（分别为23.4±10%和25.8±8.8%）。对甲苯胺蓝染色的横向切片进行光学显微镜分析显示，在陶瓷骨移植材料周围及内部均有新骨生长。这些结果表明，使用胶原 - 双相磷酸钙陶瓷与自体骨的混合物（3:1）可提供一种合适的骨传导替代物，以替代自体骨的使用，并能形成机械性能良好的融合块，与单独使用自体骨所获得的融合块无显著差异。
