Xiang Zhenghua, Burnstock Geoffrey
Department of Biochemistry and Molecular Biology, Second Military Medical University, 200433, Shanghai, People's Republic of China.
Histochem Cell Biol. 2005 Nov;124(5):379-90. doi: 10.1007/s00418-005-0043-7. Epub 2005 Nov 3.
The distribution of P2Y2 receptor-immunoreactive (ir) neurons and fibers and coexistence of P2Y2 with P2X2 and P2X3 receptors, neuropeptide Y (NPY), calretinin (CR), calbindin (CB) and nitric oxide synthase (NOS) was investigated with immunostaining methods. The results showed that P2Y2-ir neurons and fibers were distributed widely in myenteric and submucous plexuses of the guinea pig stomach corpus, jejunum, ileum and colon. The typical morphology of P2Y2-ir neurons was a long process with strong positive staining on the same side of the cell body. The P2Y2-ir neurons could be Dogiel type 1. About 40-60% P2X3-ir neurons were immunoreactive for P2Y2 in the myenteric plexus and all the P2X3-ir neurons expressed the P2Y2 receptor in the submucosal plexus; almost all the NPY-ir neurons and the majority of CR-ir neurons were also immunoreactive for P2Y2, especially in the myenteric plexus of the small intestine; no P2Y2-ir neurons were immunoreactive for P2X2 receptors, CB and NOS. It is shown for the first time that S type/Dogiel type 1 neurons with fast P2X and slow P2Y receptor-mediated depolarizations could be those neurons expressing both P2Y2-ir and P2X3-ir and that they are widely distributed in myenteric and submucosal plexuses of guinea pig gut.
采用免疫染色方法研究了P2Y2受体免疫反应性（ir）神经元和纤维的分布，以及P2Y2与P2X2、P2X3受体、神经肽Y（NPY）、钙视网膜蛋白（CR）、钙结合蛋白（CB）和一氧化氮合酶（NOS）的共存情况。结果显示，P2Y2-ir神经元和纤维广泛分布于豚鼠胃体、空肠、回肠和结肠的肌间神经丛和黏膜下神经丛。P2Y2-ir神经元的典型形态是有一个长突起，在细胞体同侧有强阳性染色。P2Y2-ir神经元可能是第1型多极神经元。在肌间神经丛中，约40%-60%的P2X3-ir神经元对P2Y2呈免疫反应，在黏膜下神经丛中所有P2X3-ir神经元均表达P2Y2受体；几乎所有NPY-ir神经元和大多数CR-ir神经元对P2Y2也呈免疫反应，尤其是在小肠的肌间神经丛中；没有P2Y2-ir神经元对P2X2受体、CB和NOS呈免疫反应。首次表明，具有快速P2X和缓慢P2Y受体介导的去极化的S型/第1型多极神经元可能是那些同时表达P2Y2-ir和P2X3-ir的神经元，并且它们广泛分布于豚鼠肠道的肌间神经丛和黏膜下神经丛中。
