在固定样品的高场双共振和三共振固态核磁共振实验中的脊髓调制去耦
SPINAL modulated decoupling in high field double- and triple-resonance solid-state NMR experiments on stationary samples.
作者信息
Sinha Neeraj, Grant Christopher V, Wu Chin H, De Angelis Anna A, Howell Stanley C, Opella Stanley J
机构信息
Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, 0307 La Jolla, CA 92093-0307, USA.
出版信息
J Magn Reson. 2005 Dec;177(2):197-202. doi: 10.1016/j.jmr.2005.07.008. Epub 2005 Aug 30.
Continuous wave irradiation has limited bandwidth for heteronuclear 1H decoupling at high fields and for 13C decoupling in 1H/13C/15N triple-resonance experiments. SPINAL-16 modulation is shown to improve the efficiency of 1H and 13C heteronuclear decoupling on single crystals of peptides and on magnetically aligned samples of membrane proteins in bicelles, which is of particular importance because aqueous samples of biomolecules are lossy at high fields, which limits the strengths of the RF fields that can be applied.
在高场下，连续波照射对于异核¹H去耦以及¹H/¹³C/¹⁵N三共振实验中的¹³C去耦而言，带宽有限。结果表明，SPINAL - 16调制可提高肽单晶以及双分子层中膜蛋白磁取向样品上¹H和¹³C异核去耦的效率，这一点尤为重要，因为生物分子的水性样品在高场下有损耗，这限制了可施加的射频场强度。
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