Rami A, Rabié A, Thomasset M, Krieglstein J
Institute of Pharmacology and Toxicology, Marburg, Germany.
J Neurosci Res. 1992 Jan;31(1):89-95. doi: 10.1002/jnr.490310113.
An antibody against rat calbindin-D28K, a calcium-binding protein present at high concentration in certain neurons of the central and peripheral nervous systems, was used to determine the progression of the pathological events in the rat hippocampus following experimental cerebral ischemia. Calbindin-D28K immunoreactivity is present in dentate granule cells and in the CA1-CA2 pyramidal cells. CA1 subfield contains a higher proportion of calbindin-D28K-positive pyramidal cells than does the CA2 subfield and CA1 cells are more immunoreactive than the CA2 cells. The pyramidal cells of the CA1 and CA2 subfields are vulnerable to ischemia. The cells in the CA1 became necrotic within 3-4 days after ischemia while those of the CA2 became necrotic within 2 days. There was a concomitant decrease in calbindin-D28K immunoreactivity in the whole hippocampal regio superior after ischemia which peaked 3 days postischemia. The difference in CA2 and CA1 vulnerability seemed to be inversely correlated with the calbindin-D28K contents of the CA2 and CA1 pyramidal cells. The decrease in the calbindin-D28K contents of these neurons was accompanied by cell damage. We therefore suggest that calbindin-D28K is an important factor for the survival of pyramidal cells in the hippocampal formation after ischemia.
一种针对大鼠钙结合蛋白-D28K的抗体被用于确定实验性脑缺血后大鼠海马体中病理事件的进展。钙结合蛋白-D28K是一种在中枢和外周神经系统的某些神经元中高浓度存在的钙结合蛋白。钙结合蛋白-D28K免疫反应性存在于齿状颗粒细胞和CA1-CA2锥体细胞中。与CA2亚区相比，CA1亚区含有更高比例的钙结合蛋白-D28K阳性锥体细胞，并且CA1细胞比CA2细胞具有更强的免疫反应性。CA1和CA2亚区的锥体细胞易受缺血影响。缺血后3-4天内，CA1区的细胞发生坏死，而CA2区的细胞在2天内发生坏死。缺血后整个海马体上区的钙结合蛋白-D28K免疫反应性随之降低，在缺血后3天达到峰值。CA2和CA1易损性的差异似乎与CA2和CA1锥体细胞中钙结合蛋白-D28K的含量呈负相关。这些神经元中钙结合蛋白-D28K含量的降低伴随着细胞损伤。因此，我们认为钙结合蛋白-D28K是缺血后海马结构中锥体细胞存活的重要因素。
