Eisele I, Wood I S, German A J, Hunter L, Trayhurn P
Neuroendocrine and Obesity Biology Unit, Liverpool Centre for Nutritional Genomics, School of Clinical Sciences, University of Liverpool, University Clinical Departments, Liverpool L69 3GA, United Kingdom.
Horm Metab Res. 2005 Aug;37(8):474-81. doi: 10.1055/s-2005-870325.
Obesity and its associated disorders are increasing in companion animals, particularly in dogs. We have investigated whether genes encoding key adipokines, some of which are implicated in the pathologies linked to obesity, are expressed in canine adipose tissues. Using RT-PCR, mRNAs encoding the following adipokines were detected in dog white adipose tissue: adiponectin, leptin, angiotensinogen, plasminogen activator inhibitor-1, IL-6, haptoglobin, metallothionein-1 and 2, and nerve growth factor. The adipokine mRNAs were present in all fat depots examined. Fractionation of adipose tissue by collagenase digestion showed that each gene was expressed in mature adipocytes. The mRNA for TNFalpha was not evident in adipose tissue, but was detected in isolated adipocytes. Fibroblastic preadipocytes from gonadal white fat were differentiated into adipocytes in primary culture and adipokine expression examined before and after differentiation (days 0 and 11, respectively). Each adipokine gene expressed in dog white adipocytes was also expressed in the differentiated cells. These results demonstrate that dog white adipose tissue expresses major adipokine genes, expression being in the adipocytes. Investigation of adipokine production and function will provide insight into the mechanisms involved in obesity-related pathologies in dogs and serve as a model for the related human diseases.
肥胖及其相关疾病在伴侣动物中日益增多，尤其是在犬类中。我们研究了编码关键脂肪因子的基因是否在犬类脂肪组织中表达，其中一些脂肪因子与肥胖相关的病理状况有关。使用逆转录聚合酶链反应（RT-PCR），在犬类白色脂肪组织中检测到了编码以下脂肪因子的信使核糖核酸（mRNA）：脂联素、瘦素、血管紧张素原、纤溶酶原激活物抑制剂-1、白细胞介素-6、触珠蛋白、金属硫蛋白-1和2以及神经生长因子。在所检查的所有脂肪库中均存在脂肪因子mRNA。通过胶原酶消化对脂肪组织进行分级分离表明，每个基因在成熟脂肪细胞中均有表达。肿瘤坏死因子α（TNFalpha）的mRNA在脂肪组织中不明显，但在分离的脂肪细胞中被检测到。来自性腺白色脂肪的成纤维前脂肪细胞在原代培养中分化为脂肪细胞，并在分化前后（分别为第0天和第11天）检测脂肪因子表达。在犬类白色脂肪细胞中表达的每个脂肪因子基因在分化细胞中也有表达。这些结果表明，犬类白色脂肪组织表达主要的脂肪因子基因，且表达位于脂肪细胞中。对脂肪因子产生和功能的研究将为了解犬类肥胖相关病理状况的机制提供见解，并可作为相关人类疾病的模型。
