Komatsuda A, Wakui H, Imai H, Nakamoto Y, Miura A B, Itoh H, Tashima Y
Third Department of Internal Medicine, Akita University School of Medicine, Japan.
Kidney Int. 1992 May;41(5):1204-12. doi: 10.1038/ki.1992.182.
We purified the constitutive 73-kDa heat-shock protein (HSP73) from the bovine brain, and produced a specific antibody against the protein in a rabbit. On immunoblotting, the antibody cross reacted only with a protein band with a molecular mass of 73 kDa in a crude extract from normal rat kidneys, which was regarded as rat renal HSP73. The intrarenal immunohistochemical distribution of HSP73 was examined by using this antibody, on both normal rat kidneys and kidneys with puromycin aminonucleoside nephrosis. HSP73 was predominantly present in epithelial cells of the glomeruli and the tubules. In normal kidneys, HSP73 was generally localized in both the cytoplasm and the nucleus of these epithelial cells, except for proximal tubular epithelial cells. On the other hand, in kidneys with puromycin aminonucleoside nephrosis, HSP73 accumulated in the cytoplasm at a level higher than in the nucleus in association with the severity of renal dysfunction and proteinuria. These findings indicate that HSP73 is mainly expressed in glomerular and tubular epithelial cells in the kidney under a physiological condition, and that its expression changes from the nucleus to the cytoplasm under pathological conditions such as a protein overload to these epithelial cells.
我们从牛脑中纯化了组成型73-kDa热休克蛋白（HSP73），并在兔体内制备了针对该蛋白的特异性抗体。在免疫印迹中，该抗体仅与正常大鼠肾脏粗提物中一条分子量为73 kDa的蛋白条带发生交叉反应，该条带被认为是大鼠肾脏HSP73。使用该抗体对正常大鼠肾脏和嘌呤霉素氨基核苷肾病大鼠肾脏进行HSP73的肾内免疫组化分布研究。HSP73主要存在于肾小球和肾小管的上皮细胞中。在正常肾脏中，除近端肾小管上皮细胞外，HSP73通常定位于这些上皮细胞的细胞质和细胞核中。另一方面，在嘌呤霉素氨基核苷肾病大鼠肾脏中，HSP73在细胞质中的积累水平高于细胞核，且与肾功能障碍和蛋白尿的严重程度相关。这些发现表明，HSP73在生理条件下主要在肾脏的肾小球和肾小管上皮细胞中表达，并且在诸如这些上皮细胞蛋白质过载等病理条件下，其表达从细胞核转移至细胞质。
