Sakuma Michiko, Akahira Jun-ichi, Suzuki Takashi, Inoue Satoshi, Ito Kiyoshi, Moriya Takuya, Sasano Hironobu, Okamura Kunihiro, Yaegashi Nobuo
Department of Obstetrics and Gynecology, Tohoku University Graduate School of Medicine, 1-1 Seiryo-machi, Aoba-ku, Sendai 980-8574, Japan.
Gynecol Oncol. 2005 Dec;99(3):664-70. doi: 10.1016/j.ygyno.2005.07.103. Epub 2005 Sep 2.
The estrogen-responsive ring finger protein (Efp) gene, one of estrogen receptor (ER) target genes, is considered to be essential for estrogen-dependent cell proliferation. To understand the estrogenic action on ovarian cancer, we studied the relationships between Efp and ERs expressions and the correlations of Efp expression with clinicopathological parameters in epithelial ovarian cancer.
The protein expressions for Efp, ERalpha and ERbeta were examined by immunoblotting in 12 ovarian cancer cell lines. Efp mRNA expressions were evaluated by quantitative RT-PCR in 12 ovarian cancer cell lines. A total of 100 surgical specimens diagnosed as epithelial ovarian cancer were examined immunohistochemically using antibodies for Efp, ERalpha and ERbeta.
Efp protein was detected in 8 out of 12 cell lines. In Efp protein-positive cell lines, Efp mRNA was expressed higher than that in negative (P=0.021). All of the Efp protein-positive cell lines simultaneously expressed either ERalpha or ERbeta protein. By immunohistochemical staining, Efp immunoreactivity was detected in 63 out of 100 ovarian cancer specimens and positive signals were in the cytoplasm of carcinoma cells. There were significant correlations between Efp and ERalpha, ERbeta immunoreactivity (Efp and ERalpha, P=0.022; Efp and ERbeta, P=0.032). Efp expression was significantly higher in a subgroup with serous adenocarcinoma (P=0.010) and with advanced disease (P=0.026). No significant relationship was detected between Efp immunoreactivity and overall survival.
The expression of Efp was detected in human epithelial ovarian cancer and high expression of Efp was correlated with advanced disease and serous adenocarcinoma, and ERs status.
雌激素反应性泛素连接酶（Efp）基因作为雌激素受体（ER）的靶基因之一，被认为对雌激素依赖性细胞增殖至关重要。为了解雌激素对卵巢癌的作用，我们研究了Efp与ERs表达之间的关系以及Efp表达与上皮性卵巢癌临床病理参数的相关性。
通过免疫印迹法检测12种卵巢癌细胞系中Efp、ERα和ERβ的蛋白表达。采用定量逆转录聚合酶链反应（RT-PCR）评估12种卵巢癌细胞系中Efp mRNA的表达。使用Efp、ERα和ERβ抗体对100例诊断为上皮性卵巢癌的手术标本进行免疫组织化学检测。
12种细胞系中有8种检测到Efp蛋白。在Efp蛋白阳性的细胞系中，Efp mRNA的表达高于阴性细胞系（P=0.021）。所有Efp蛋白阳性的细胞系均同时表达ERα或ERβ蛋白。通过免疫组织化学染色，在100例卵巢癌标本中有63例检测到Efp免疫反应性，阳性信号位于癌细胞的细胞质中。Efp与ERα、ERβ免疫反应性之间存在显著相关性（Efp与ERα，P=0.022；Efp与ERβ，P=0.032）。浆液性腺癌亚组（P=0.010）和晚期疾病亚组（P=0.026）中Efp的表达明显更高。未检测到Efp免疫反应性与总生存期之间存在显著关系。
在人上皮性卵巢癌中检测到Efp的表达，Efp的高表达与晚期疾病、浆液性腺癌以及ERs状态相关。
