共聚焦显微镜检查：宫颈癌前病变成像
Confocal microscopy: imaging cervical precancerous lesions.
作者信息
Carlson Kristen, Pavlova Ina, Collier Tom, Descour Michael, Follen Michele, Richards-Kortum Rebecca
机构信息
Department of Biomedical Engineering, 1 University Station, C0800, The University of Texas at Austin, Austin, TX 78712, USA.
出版信息
Gynecol Oncol. 2005 Dec;99(3 Suppl 1):S84-8. doi: 10.1016/j.ygyno.2005.07.049. Epub 2005 Sep 6.
OBJECTIVES
We explore the clinical potential of reflectance and fluorescence confocal microscopy to image the morphologic and biochemical changes associated with precancer, in order to aid in the detection and diagnosis of cervical dysplasia.
METHODS
Cervical epithelial tissue samples imaged ex vivo or in vivo were obtained from M. D. Anderson Cancer Center and Lyndon B. Johnson Hospital in Houston, Texas. Confocal reflectance microscopy was used to image ex vivo cervical biopsies and in vivo cervical tissue. Confocal fluorescence microscopy was used to image ex vivo cervical tissue slices.
RESULTS
We present reflectance and fluorescence confocal images of cervical tissue demonstrating the ability to differentiate between normal and abnormal cervical tissue.
CONCLUSIONS
We believe that there is significant clinical potential for confocal microscopy to provide a sensitive and specific method for cervical precancer detection.
目的
我们探索反射共聚焦显微镜和荧光共聚焦显微镜在对与癌前病变相关的形态学和生化变化进行成像方面的临床潜力，以辅助宫颈发育异常的检测和诊断。
方法
从德克萨斯州休斯顿的MD安德森癌症中心和林登·B·约翰逊医院获取离体或在体成像的宫颈上皮组织样本。共聚焦反射显微镜用于对离体宫颈活检组织和在体宫颈组织进行成像。共聚焦荧光显微镜用于对离体宫颈组织切片进行成像。
结果
我们展示了宫颈组织的反射共聚焦图像和荧光共聚焦图像，证明其能够区分正常和异常宫颈组织。
结论
我们认为共聚焦显微镜在提供一种用于宫颈癌前病变检测的灵敏且特异的方法方面具有巨大的临床潜力。
Zotero 插件