Linxweiler Maximilian, Kadah Basel Al, Bozzato Alessandro, Bozzato Victoria, Hasenfus Andrea, Kim Yoo-Jin, Wagner Mathias, Igressa Alhadi, Schick Bernhard, Charalampaki Patra
Department of Otorhinolaryngology, Head and Neck Surgery, Saarland University Medical Center, Kirrbergerstr. 100, building 6, 66421, Homburg/Saar, Germany.
Department of General and Surgical Pathology, Saarland University Medical Center, Kirrbergerstr. 100, 66421, Homburg/Saar, Germany.
Eur Arch Otorhinolaryngol. 2016 Dec;273(12):4473-4483. doi: 10.1007/s00405-016-4145-8. Epub 2016 Jun 15.
Confocal laser endomicroscopy (CLE) is an imaging technique that uses miniaturized fiberoptic probes to allow real-time histological imaging of human tissue. An application of CLE in otorhinolaryngology has hardly been investigated so far. In our study, we analyzed the applicability of CLE to visualize cancerous and healthy tissue of the head and neck region. Formalin-fixed tissue specimens from 135 head and neck squamous cell carcinoma (HNSCC) patients and 50 healthy controls were investigated using CLE with and without topical application of acriflavine. Four head and neck surgeons, four pathologists, and four laymen evaluated the CLE images of the HNSCC cases regarding the tumor localization and its border to healthy tissue. The tumor localization and the tumor border were correctly identified in 97 % by the pathologists, 85 % by the head and neck surgeons, and 70 % by the laymen. The main difference in evaluation results was seen in the correct identification of the tumor site (p < 0.05), while there was no significant difference in the identification of the tumor border. CLE is a valuable tool for real-time histological imaging of HNSCCs. It can help to visualize the tumor border and, thereby, facilitate a more precise tumor surgery.
共聚焦激光内镜检查（CLE）是一种成像技术，它使用小型化光纤探头对人体组织进行实时组织学成像。到目前为止，CLE在耳鼻咽喉科的应用几乎未被研究过。在我们的研究中，我们分析了CLE用于可视化头颈部区域癌组织和健康组织的适用性。使用CLE并在局部应用吖啶黄的情况下，对135名头颈部鳞状细胞癌（HNSCC）患者和50名健康对照的福尔马林固定组织标本进行了研究。四名头颈外科医生、四名病理学家和四名外行人对HNSCC病例的CLE图像进行了评估，内容涉及肿瘤定位及其与健康组织的边界。病理学家正确识别肿瘤定位和肿瘤边界的比例为97%，头颈外科医生为85%，外行为70%。评估结果的主要差异体现在肿瘤部位的正确识别上（p<0.05），而在肿瘤边界的识别上没有显著差异。CLE是用于HNSCC实时组织学成像的有价值工具。它有助于可视化肿瘤边界，从而促进更精确的肿瘤手术。
