鼓室内应用地塞米松后耳蜗的结构与功能影响

[Structure and function influence of cochlear after dexamethasone intratympanic application].

作者信息

Liu Hong-jian, Dong Ming-min, Chi Fang-lu

机构信息

Department of Otorhinolaryngology, Eye Ear Nose & Throat Hospital, Fudan University, Shanghai 200031, China.

出版信息

Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2005 Jun;40(6):440-3.

PMID:16144342

Abstract

OBJECTIVE

To study the changes of the structure and function of cochlear after dexamethasone intratympanic application.

METHODS

Forty-five guinea pigs were divided into three groups as normal group, 0.9% sodium chloride injection group and 0.5% dexamethasone group. By using the auditory brainstem response (ABR), the superoxide dismutase (SOD) activity, the nitric oxide (NO) value and cochlear surface preparation technique, the changes of structure and function of cochlear after intratympanic Dexamethasone application has been investigated in this study.

RESULTS

There was not significant difference among three groups in ABR thresholds of wave III (F = 0.5, P = 0.5). There was not significant difference among three groups in SOD activity (F = 2.45, P = 0.96). There was not significant difference among three groups in NO value (F = 3.1, P = 0.3). There was not significant difference among three groups in necrotic values of hair cells on the cochlear surface specimens in 15 mm from basal turn (F = 0.93, P = 0.45).

CONCLUSION

There is no change of cochlear construction and function after intratympanic Dexamethasone application.

摘要

目的

研究鼓室内应用地塞米松后耳蜗结构和功能的变化。

方法

将45只豚鼠分为正常组、0.9%氯化钠注射液组和0.5%地塞米松组。本研究采用听性脑干反应（ABR）、超氧化物歧化酶（SOD）活性、一氧化氮（NO）值及耳蜗表面制备技术，研究鼓室内应用地塞米松后耳蜗结构和功能的变化。

结果

三组在Ⅲ波ABR阈值上差异无统计学意义（F = 0.5，P = 0.5）。三组在SOD活性上差异无统计学意义（F = 2.45，P = 0.96）。三组在NO值上差异无统计学意义（F = 3.1，P = 0.3）。三组在距蜗底15 mm处耳蜗表面标本毛细胞坏死值上差异无统计学意义（F = 0.93，P = 0.45）。