三种不同聚合酶链反应方法用于定量宫颈刮片标本中16型人乳头瘤病毒DNA的比较
Comparison of three different PCR methods for quantifying human papillomavirus type 16 DNA in cervical scrape specimens.
作者信息
Hesselink A T, van den Brule A J C, Groothuismink Z M A, Molano M, Berkhof J, Meijer C J L M, Snijders P J F
机构信息
Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands.
出版信息
J Clin Microbiol. 2005 Sep;43(9):4868-71. doi: 10.1128/JCM.43.9.4868-4871.2005.
Abstract
We compared real-time LightCycler and TaqMan assays and the GP5+/6+ PCR/enzyme immunoassay (EIA) to assess the human papillomavirus type 16 (HPV16) load in cervical scrape specimens. Both real-time PCR assays determined the HPV16 load in scrape specimens similarly. The level of agreement between these assays and the GP5+/6+ PCR/EIA was low (P = 0.004), suggesting that the latter method is not suited for quantifying HPV16 DNA.
摘要
我们比较了实时荧光定量PCR仪和TaqMan检测法以及GP5+/6+聚合酶链反应/酶免疫测定法（EIA），以评估宫颈刮片标本中16型人乳头瘤病毒（HPV16）的载量。两种实时荧光定量PCR检测法对刮片标本中HPV16载量的测定结果相似。这些检测法与GP5+/6+聚合酶链反应/酶免疫测定法之间的一致性水平较低（P = 0.004），表明后一种方法不适用于定量HPV16 DNA。
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