Li Li-Jun, Zhang Jian, Zhang Feng, Lineaweaver William C, Chen Tong-Yi, Chen Zhong-Wei
Department of Orthopedic Surgery, Zhong Shan Hospital, Fudan University, Shanghai, China.
Microsurgery. 2005;25(7):561-5. doi: 10.1002/micr.20159.
The purpose of this study was to evaluate the value of utilizing longitudinal intrafascicular electrodes (LIFEs) in collecting and analyzing sensory signals from the peripheral nerve. The longitudinal intrafascicular electrodes were made of 25-microm Teflon-insulated Pt/Ir wire and implanted into the fascicle of the superficial peroneal nerves in a feline model. The sensory signals at rest status and induced with various stimulations were recorded. The action potential area, frequency, coefficient of variation (CV) of the peak, and functional spectrum were then analyzed by the MF Lab version 3.01 software package. The results showed that the sensory nerve action potentials (SNAPs) were 0-2 spikes per second at rest state; the count was increased when stimulation was administered. SNAPs were 16-24 spikes per second when scraping stimulation was applied. The pulse intervals and the waveform remained consistent. SNAPs burst and were clustered when stress stimulation was given. The comparison of area, frequency, and CV of the peak showed statistically significant differences between these parameters receiving different stimulations. The functional spectrum analysis showed that the frequency of action potential increased when the stress stimulation was applied. In conclusion, LIFEs can sensitively collect sensory signals and provide a good interface to analyze sensory information from peripheral fasciculi. These data provide useful information for further study of control of electronic prostheses.
本研究的目的是评估使用纵向束内电极（LIFE）收集和分析来自周围神经的感觉信号的价值。纵向束内电极由25微米聚四氟乙烯绝缘的铂/铱丝制成，并植入猫模型的腓浅神经束中。记录静息状态和各种刺激诱导下的感觉信号。然后使用MF Lab 3.01软件包分析动作电位面积、频率、峰值变异系数（CV）和功能谱。结果显示，静息状态下感觉神经动作电位（SNAP）为每秒0 - 2个峰；施加刺激时计数增加。施加刮擦刺激时，SNAP为每秒16 - 24个峰。脉冲间隔和波形保持一致。施加应激刺激时，SNAP爆发并聚集。峰值面积、频率和CV的比较显示，接受不同刺激的这些参数之间存在统计学显著差异。功能谱分析表明，施加应激刺激时动作电位频率增加。总之，LIFE可以灵敏地收集感觉信号，并为分析来自周围神经束的感觉信息提供良好的界面。这些数据为进一步研究电子假体的控制提供了有用的信息。
