Haswell-Elkins M R, Leonard H, Kennedy M W, Elkins D B, Maizels R M
Tropical Health Program, Queensland Institute of Medical Research, Brisbane, Australia.
Parasitology. 1992 Feb;104 Pt 1:153-9. doi: 10.1017/s0031182000060893.
The serum antibody responses of 124 people naturally exposed to Ascaris lumbricoides infection were analysed by immunoprecipitation of radio-isotope labelled 3rd- and 4th-stage larval Ascaris suum excretory and secretory antigens (L3/4 ES). Profiles of antigens recognized were visualized by polyacrylamide gel electrophoresis (SDS-PAGE), and the band intensities of the 12 major precipitated antigens were individually scored. Most subjects were seropositive, but considerable variation was observed in the amount of total and individual ES antigens precipitated. The sex- and age-related profiles of antibody levels followed similar patterns to those of egg output. In addition, total antibody scores of individuals were closely correlated (r = 0.47-0.52) with their eggs per gram of faeces (e.p.g.) collected 4 months after blood samples were taken. These findings suggest that antibody levels against larval ES antigens reflect recent exposure and are consistent with the hypothesis that establishment of adult worms is proportional to the number of larvae that recently migrated through the lung.
通过对放射性同位素标记的猪蛔虫第三和第四阶段幼虫排泄及分泌抗原（L3/4 ES）进行免疫沉淀，分析了124名自然暴露于蛔虫感染人群的血清抗体反应。通过聚丙烯酰胺凝胶电泳（SDS-PAGE）观察所识别抗原的图谱，并对12种主要沉淀抗原的条带强度分别进行评分。大多数受试者血清呈阳性，但在沉淀的总ES抗原和个体ES抗原量方面观察到相当大的差异。抗体水平的性别和年龄相关图谱与虫卵排出量的图谱遵循相似模式。此外，个体的总抗体评分与采血4个月后每克粪便中的虫卵数（e.p.g.）密切相关（r = 0.47 - 0.52）。这些发现表明，针对幼虫ES抗原的抗体水平反映了近期的暴露情况，并且与成虫建立与近期通过肺部迁移的幼虫数量成正比这一假设相一致。
