Urban J F, Romanowski R D
Exp Parasitol. 1985 Oct;60(2):245-54. doi: 10.1016/0014-4894(85)90028-1.
Parasite products were collected at three distinct phases of development of Ascaris suum, and their immunogenicity was determined after injection into rabbits and pigs. Products were derived from (1) the hatching fluid of infective eggs; (2) the conditioned medium of 2nd-stage larvae that developed to 3rd stage in vitro in defined medium; and (3) the conditioned medium of 3rd-stage larvae that developed to 4th stage in vitro in defined medium. Protein profiles from these three preparations, separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, were less complex than that of extracts from homogenized A. suum larvae. Hyperimmune rabbit antiserum raised against either egg products, 2nd- to 3rd-stage larval excretory-secretory products, or 3rd- to 4th-stage larval excretory-secretory products showed strong homologous reactions after immunoelectrophoresis, but relatively weak cross-reactions with the other preparations. A combined enteral immunization of pigs with egg products and parenteral immunization with the 2nd- to 3rd-stage larval excretory-secretory products, and 3rd- to 4th-stage larval excretory-secretory products induced antibody to each preparation and significant protective immunity to a challenge exposure with 10,000 A. suum eggs. However, a marked pathological response to larvae migrating in the liver after challenge exposure was also induced.
收集猪蛔虫发育三个不同阶段的寄生虫产物，并将其注射到兔子和猪体内后测定其免疫原性。产物来源于：（1）感染性虫卵的孵化液；（2）在特定培养基中体外发育至第三阶段的二期幼虫的条件培养基；（3）在特定培养基中体外发育至第四阶段的三期幼虫的条件培养基。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离的这三种制剂的蛋白质谱，比猪蛔虫幼虫匀浆提取物的蛋白质谱更简单。用虫卵产物、二期至三期幼虫排泄分泌产物或三期至四期幼虫排泄分泌产物制备的超免疫兔抗血清在免疫电泳后显示出强烈的同源反应，但与其他制剂的交叉反应相对较弱。用虫卵产物对猪进行联合肠内免疫，并用二期至三期幼虫排泄分泌产物和三期至四期幼虫排泄分泌产物进行肠外免疫，可诱导针对每种制剂的抗体，并对10000个猪蛔虫卵的攻击暴露产生显著的保护性免疫。然而，在攻击暴露后，也诱导了对在肝脏中迁移的幼虫的明显病理反应。
