大鼠脑缺血后海马祖细胞表达巢蛋白。

Hippocampal progenitor cells express nestin following cerebral ischemia in rats.

作者信息

He Zhen, Cui Li, Meschia James F, Dickson Dennis W, Brott Thomas G, Simpkins James W, Day Arthur L, McKinney Michael

机构信息

Department of Pharmacology, Mayo Clinic Jacksonville, Jacksonville, Florida 32224, USA.

出版信息

Neuroreport. 2005 Sep 28;16(14):1541-4. doi: 10.1097/01.wnr.0000179074.32035.46.

DOI:10.1097/01.wnr.0000179074.32035.46

PMID:16148741

Abstract

This study addresses whether hippocampal progenitor cells express nestin following cerebral ischemia in rats. Cell counts within the hippocampal hilus were significantly greater following severe (eight-vessel occlusion) ischemia than following intermediate (four-vessel occlusion) ischemia (1527+/-87/mm2 vs. 918+/-71/mm2). Bromedeoxyuridine-positive cell counts were significantly higher with severe ischemia than with intermediate ischemia or in sham-operated animals, respectively (368+/-45, 43+/-14 and 7+/-1/mm2). In the eight-vessel occlusion group, 47+/-8/mm2 bromedeoxyuridine-labeled cells expressed nestin, significantly higher than in the four-vessel occlusion group and sham-operated animals (1+/-1 and 1+/-0/mm2, P<0.01 vs. eight-vessel occlusion, respectively). Confocal microscopy verified that a subset of the bromedeoxyuridine-positive cells expressed nestin. In conclusion, severe ischemia elicits nestin expression in hippocampal progenitor cells in rats.

摘要

本研究探讨大鼠脑缺血后海马祖细胞是否表达巢蛋白。重度（八动脉闭塞）缺血后海马齿状回内的细胞计数显著高于中度（四动脉闭塞）缺血后（1527±87/mm² 对 918±71/mm²）。重度缺血组溴脱氧尿苷阳性细胞计数分别显著高于中度缺血组或假手术动物组（368±45、43±14 和 7±1/mm²）。在八动脉闭塞组中，47±8/mm² 的溴脱氧尿苷标记细胞表达巢蛋白，显著高于四动脉闭塞组和假手术动物组（1±1 和 1±0/mm²，与八动脉闭塞组相比 P<0.01）。共聚焦显微镜证实，部分溴脱氧尿苷阳性细胞表达巢蛋白。总之，重度缺血可诱导大鼠海马祖细胞表达巢蛋白。