Ren Xiu-jun, Ma Hui-fang
School of Acu-moxibustion, Beijing University of Chinese Medicine, Beijing 100029, China.
Zhen Ci Yan Jiu. 2010 Jun;35(3):175-81.
To observe the effect of electroacupuncture (EA) and acupuncture (A) on the proliferation of stem cells in the subependymal zone (SPZ) of the lateral ventricle and the frontal lobe cortex in hyperlipemia(HL) combined with cerebral ischemia (CI) rats.
A total of 72 male SD rats were randomized into control, HL, HL+EA, CI, CI+A, HL+CI, HL+CI+EA I and HL+CI+EA II groups (n=9 /group). HL model was established by feeding the animals with high fat forage for 6 weeks and CI model was established by FeCl3-induced occlusion of the unilateral middle cerebral artery. EA was applied to "Sanyinjiao" (SP 6) and "Fenglong" (ST 40) once daily for 17 days for HL+ EA group; and acupuncture to "Baihui" (GV 20) and "Shuigou" (GV 26) once daily for 7 days for CI + A group. For HL+CI+EA I group, EA was applied to SP 6 + ST 40 first before CI, once daily for 10 days, followed by EA of SP 6+ST 40 and acupuncture of GV20+GV26 for 7 days after CI. For HL+CI+EA II group, no treatment was given before CI, then, acupuncture of GV 20 + GV 26 and EA of SP 6 + ST 40 were given once daily for 7 days after CI. The immunoactivity of Nestin and proliferation cell nuclear antigen (PONA) of SPZ was detected by immunohistochemistry.
In comparison with normal control group, the numbers of both Nestin and PCNA immunoreaction (IR) positive cells in the dorsolateral extension and the wall of the lateral ventricle of the brain increased significantly in CI and HL+CI groups (P < 0.01). Compared with CI group, the numbers of Nestin and PCNA IR positive cells in the dorsolateral extension and the wall of the lateral ventricle in CI + A group increased considerably (P < 0.01). In comparison with HL+CI group, both Nestin and PCNA IR positive cell numbers in the dorsolateral extension and the wall of the lateral ventricle of the brain in HL+CI+EA I and HL+CI+EA II groups increased significantly (P < 0.01), and the effect of HL+CI+EA I group was markedly superior to that of HL+CI+EA II group in upregulating the numbers of Nestin and PCNA IR positive cells in the aforementioned regions of the lateral ventricle (P < 0.01). No significant differences were found between HL and control groups, and between HL+EA and HL groups in the numbers of Nestin and PCNA IR positive cells in the dorsolateral extension and the wall of the lateral ventricle of the brain (P > 0.05).
EA can upregulate Nestin and PCNA expression of the dorsolateral extension and the wall of the lateral ventricle of the brain on the ischemic side in rats with CI, and with HL+CI, which may contribute to its effects in promoting the proliferation and migration of neural stem cells in the brain.
观察电针（EA）与针刺（A）对高脂血症（HL）合并脑缺血（CI）大鼠侧脑室室管膜下区（SPZ）及额叶皮质干细胞增殖的影响。
将72只雄性SD大鼠随机分为对照组、HL组、HL+EA组、CI组、CI+A组、HL+CI组、HL+CI+EAⅠ组和HL+CI+EAⅡ组（每组n=9）。通过喂养高脂饲料6周建立HL模型，采用FeCl3诱导阻断单侧大脑中动脉建立CI模型。HL+EA组每日针刺“三阴交”（SP 6）和“丰隆”（ST 40）1次，共17天；CI+A组每日针刺“百会”（GV 20）和“水沟”（GV 26）1次，共7天。HL+CI+EAⅠ组在CI前先针刺SP 6+ST 40，每日1次，共10天，CI后针刺SP 6+ST 40及GV20+GV26共7天。HL+CI+EAⅡ组在CI前不做处理，CI后每日针刺GV 20+GV 26及SP 6+ST 40共7天。采用免疫组化法检测SPZ中巢蛋白（Nestin）和增殖细胞核抗原（PCNA）的免疫活性。
与正常对照组比较，CI组和HL+CI组大鼠脑外侧背侧延伸部及侧脑室壁Nestin和PCNA免疫反应（IR）阳性细胞数显著增加（P<0.01）。与CI组比较，CI+A组大鼠脑外侧背侧延伸部及侧脑室壁Nestin和PCNA IR阳性细胞数明显增加（P<0.01）。与HL+CI组比较，HL+CI+EAⅠ组和HL+CI+EAⅡ组大鼠脑外侧背侧延伸部及侧脑室壁Nestin和PCNA IR阳性细胞数均显著增加（P<0.01），且HL+CI+EAⅠ组上调侧脑室上述区域Nestin和PCNA IR阳性细胞数的作用明显优于HL+CI+EAⅡ组（P<0.01）。HL组与对照组、HL+EA组与HL组大鼠脑外侧背侧延伸部及侧脑室壁Nestin和PCNA IR阳性细胞数比较，差异无统计学意义（P>0.05）。
电针可上调CI大鼠及HL+CI大鼠缺血侧脑外侧背侧延伸部及侧脑室壁Nestin和PCNA的表达，这可能是其促进脑内神经干细胞增殖和迁移的作用机制之一。
