Afshin Salimeh, Keshavarz Mansoor, Salami Mahmood, Mirershadi Fatemeh, Djahanguiri Bijan
Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
World J Gastroenterol. 2005 Sep 14;11(34):5358-61. doi: 10.3748/wjg.v11.i34.5358.
To study the effects of indomethacin on the isolated transverse and longitudinal rat gastric fundus strips.
The strips were suspended in an organ bath containing oxygenated Krebs solution, and contractile responses to electrical field stimulation were recorded on a physiograph in an isotonic manner after administration of cumulative concentrations of indomethacin. The effects of indomethacin on the strips pretreated with K(ATP) channel modulators, diazoxide and glybenclamide were studied.
Treatment of the transverse strips with indomethacin resulted in a concentration-dependent inhibitory response. In longitudinal strips, biphasic responses were seen, which included a stimulatory response at low concentrations of indomethacin, followed by an inhibitory response at higher concentrations. Diazoxide pre-treatment inhibited the stimulatory response of longitudinal strips. Glybenclamide pre-treatment not only blocked inhibitory effect of the low concentrations of indomethacin on transverse strips, but also increased the amplitude of contractions. Moreover, the drug decreased the amplitude of contractions in longitudinal strips.
Responses of the isolated longitudinal and transverse rat gastric fundus strips to indomethacin are not similar, and are influenced by K(ATP) channel modulators.
研究吲哚美辛对离体大鼠胃底横条和纵条的作用。
将条带悬挂于含有充氧Krebs溶液的器官浴中，在给予累积浓度的吲哚美辛后，以等张方式在生理记录仪上记录对电场刺激的收缩反应。研究了吲哚美辛对用K(ATP)通道调节剂二氮嗪和格列本脲预处理的条带的影响。
用吲哚美辛处理横条产生浓度依赖性抑制反应。在纵条中，观察到双相反应，包括低浓度吲哚美辛时的刺激反应，随后是高浓度时的抑制反应。二氮嗪预处理抑制了纵条的刺激反应。格列本脲预处理不仅阻断了低浓度吲哚美辛对横条的抑制作用，还增加了收缩幅度。此外，该药物降低了纵条的收缩幅度。
离体大鼠胃底纵条和横条对吲哚美辛的反应不相似，且受K(ATP)通道调节剂影响。
