Yurkin Maxim A, Semyanov Konstantin A, Tarasov Peter A, Chernyshev Andrei V, Hoekstra Alfons G, Maltsev Valeri P
Institute of Chemical Kinetics and Combustion, Siberian Branch of the Russian Academy of Sciences, Institutskaya 3, Novosibirsk 630090, Russia.
Appl Opt. 2005 Sep 1;44(25):5249-56. doi: 10.1364/ao.44.005249.
Elastic light scattering by mature red blood cells (RBCs) was theoretically and experimentally analyzed by use of the discrete dipole approximation (DDA) and scanning flow cytometry (SFC), respectively. SFC permits measurement of the angular dependence of the light-scattering intensity (indicatrix) of single particles. A mature RBC is modeled as a biconcave disk in DDA simulations of light scattering. We have studied the effect of RBC orientation related to the direction of the light incident upon the indicatrix. Numerical calculations of indicatrices for several axis ratios and volumes of RBC have been carried out. Comparison of the simulated indicatrices and indicatrices measured by SFC showed good agreement, validating the biconcave disk model for a mature RBC. We simulated the light-scattering output signals from the SFC with the DDA for RBCs modeled as a disk-sphere and as an oblate spheroid. The biconcave disk, the disk-sphere, and the oblate spheroid models have been compared for two orientations, i.e., face-on and rim-on incidence, relative to the direction of the incident beam. Only the oblate spheroid model for rim-on incidence gives results similar to those of the rigorous biconcave disk model.
分别利用离散偶极子近似法（DDA）和扫描流式细胞术（SFC），从理论和实验两方面对成熟红细胞（RBC）的弹性光散射进行了分析。SFC能够测量单个粒子光散射强度（光强分布曲线）的角度依赖性。在光散射的DDA模拟中，将成熟红细胞建模为双凹盘。我们研究了红细胞取向对光强分布曲线的影响，其中红细胞取向与光入射方向相关。已对几种轴比和体积的红细胞的光强分布曲线进行了数值计算。模拟的光强分布曲线与SFC测量的光强分布曲线的比较显示出良好的一致性，验证了成熟红细胞的双凹盘模型。我们用DDA对建模为盘球体和扁球体的红细胞的SFC光散射输出信号进行了模拟。已针对相对于入射光束方向的两种取向，即正面入射和边缘入射，对双凹盘、盘球体和扁球体模型进行了比较。只有边缘入射的扁球体模型给出的结果与严格的双凹盘模型相似。
