成熟红细胞：从光学模型到逆光散射问题
Mature red blood cells: from optical model to inverse light-scattering problem.
作者信息
Gilev Konstantin V, Yurkin Maxim A, Chernyshova Ekaterina S, Strokotov Dmitry I, Chernyshev Andrei V, Maltsev Valeri P
机构信息
Voevodsky Institute of Chemical Kinetics and Combustion SB RAS, Institutskaya 3, 630090, Novosibirsk, Russia; Novosibirsk State University, Pirogova 2, 630090, Novosibirsk, Russia.
Voevodsky Institute of Chemical Kinetics and Combustion SB RAS, Institutskaya 3, 630090, Novosibirsk, Russia; Novosibirsk State Medical University, Krasny Prospect 52, 630091, Novosibirsk, Russia.
出版信息
Biomed Opt Express. 2016 Mar 15;7(4):1305-10. doi: 10.1364/BOE.7.001305. eCollection 2016 Apr 1.
We propose a method for characterization of mature red blood cells (RBCs) morphology, based on measurement of light-scattering patterns (LSPs) of individual RBCs with the scanning flow cytometer and on solution of the inverse light-scattering (ILS) problem for each LSP. We considered a RBC shape model, corresponding to the minimal bending energy of the membrane with isotropic elasticity, and constructed an analytical approximation, which allows rapid simulation of the shape, given the diameter and minimal and maximal thicknesses. The ILS problem was solved by the nearest-neighbor interpolation using a preliminary calculated database of 250,000 theoretical LSPs. For each RBC in blood sample we determined three abovementioned shape characteristics and refractive index, which also allows us to calculate volume, surface area, sphericity index, spontaneous curvature, hemoglobin concentration and content.
我们提出了一种基于用扫描流式细胞仪测量单个红细胞的光散射模式（LSPs）以及求解每个LSP的逆光散射（ILS）问题来表征成熟红细胞（RBCs）形态的方法。我们考虑了一个与具有各向同性弹性的膜的最小弯曲能量相对应的红细胞形状模型，并构建了一个解析近似，该近似在给定直径、最小和最大厚度的情况下能够快速模拟形状。通过使用预先计算的250,000个理论LSPs的数据库进行最近邻插值来解决ILS问题。对于血样中的每个红细胞，我们确定了上述三个形状特征和折射率，这也使我们能够计算体积、表面积、球形指数、自发曲率、血红蛋白浓度和含量。
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