Krebs Desiree L, Parent Marise B
Department of Psychology and Center for Behavioral Neuroscience, Georgia State University, P. O. Box 3966, Atlanta, Georgia 30302-3966, USA.
Eur J Pharmacol. 2005 Sep 27;520(1-3):91-9. doi: 10.1016/j.ejphar.2005.08.007.
Hippocampal infusions of glucose reverse memory deficits in spontaneous alternation and in a continuous multiple trial inhibitory avoidance task. The current experiments tested whether glucose metabolism may participate in these effects of glucose. Specifically, these experiments determined whether the glycolytic metabolite pyruvate would mimic these effects of glucose. Male Sprague-Dawley rats were given septal infusions of vehicle or the gamma-aminobutyric acid (GABA) receptor agonist muscimol (0.15 nmol for spontaneous alternation or 5 nmol for continuous multiple trial inhibitory avoidance) combined with hippocampal infusions of vehicle or pyruvate (200 nmol) 15 min prior to assessing spontaneous alternation or training in a continuous multiple trial inhibitory avoidance task. The infusions of muscimol decreased percent alternation scores and continuous multiple trial inhibitory avoidance retention latencies tested 48 h after training. More importantly, hippocampal infusions of pyruvate reversed the deficits produced by septal infusions of muscimol on both tasks. These findings show for the first time that hippocampal infusions of pyruvate influence memory and suggest that glucose may affect memory via glycolytic metabolism.
向海马体注射葡萄糖可逆转自发交替任务以及连续多次试验抑制性回避任务中的记忆缺陷。当前实验测试了葡萄糖代谢是否可能参与葡萄糖的这些作用。具体而言，这些实验确定了糖酵解代谢产物丙酮酸是否会模拟葡萄糖的这些作用。在评估自发交替任务或进行连续多次试验抑制性回避任务训练前15分钟，给雄性斯普拉格-道利大鼠的中隔注入溶媒或γ-氨基丁酸（GABA）受体激动剂蝇蕈醇（自发交替任务用0.15纳摩尔，连续多次试验抑制性回避任务用5纳摩尔），并向海马体注入溶媒或丙酮酸（200纳摩尔）。注入蝇蕈醇降低了训练48小时后测试的交替百分比得分和连续多次试验抑制性回避任务的保留潜伏期。更重要的是，向海马体注入丙酮酸逆转了中隔注入蝇蕈醇在两项任务上产生的缺陷。这些发现首次表明向海马体注入丙酮酸会影响记忆，并提示葡萄糖可能通过糖酵解代谢影响记忆。
