Sajikumar Sreedharan, Navakkode Sheeja, Frey Julietta Uta
Department of Neurophysiology, Leibniz Institute for Neurobiology, Brenneckestrasse 6, 39118 Magdeburg, Germany.
Curr Opin Neurobiol. 2005 Oct;15(5):607-13. doi: 10.1016/j.conb.2005.08.009.
There is growing interest in late-LTP and late-LTD, that is, distinct forms of functional plasticity that require somatic functions such as protein synthesis in addition to the transient synaptic processes that are required for short lasting forms. Interestingly, to date only these forms of lasting plastic events could be detected in healthy, freely moving animals and thus, they are considered as physiological cellular models of learning and memory formation. Late-LTP and -LTD are characterized by 'synaptic tagging' or 'capture' and 'synaptic cross-tagging', but there are only a few laboratories that can currently perform experiments studying these properties. In brain slice work, there are many different approaches to investigate these processes using different methodological variations: some allow slices to rest for long periods before the experiment starts, others do not; some run their experiments at near to physiological temperatures, others at lower temperatures; some stimulate frequently, others do not.
人们对晚期长时程增强（late-LTP）和晚期长时程抑制（late-LTD）的兴趣与日俱增，也就是说，这是两种不同形式的功能可塑性，除了短暂形式所需的瞬时突触过程外，还需要诸如蛋白质合成等体细胞功能。有趣的是，迄今为止，只有这些形式的持久可塑性事件能够在健康的自由活动动物中被检测到，因此，它们被视为学习和记忆形成的生理细胞模型。晚期LTP和-LTD的特征是“突触标记”或“捕获”以及“突触交叉标记”，但目前只有少数实验室能够进行研究这些特性的实验。在脑片研究中，有许多不同的方法来使用不同的方法变体研究这些过程：一些方法允许脑片在实验开始前长时间静置，另一些则不允许；一些在接近生理温度下进行实验，另一些在较低温度下进行；一些频繁刺激，另一些则不刺激。
