Cárdenas Horacio, Pope William F
Department of Animal Sciences, The Ohio State University, Columbus, 43210, USA.
Domest Anim Endocrinol. 2005 Oct;29(3):523-33. doi: 10.1016/j.domaniend.2005.03.001. Epub 2005 Apr 7.
Experiments were conducted to evaluate expression of the estrogen receptor (ER) alpha and ERbeta genes in the uterus and ovarian follicles of gilts treated with 5alpha-dihydrotestosterone (DHT) during the follicular phase of the estrous cycle. This DHT treatment has enhanced ovulation rate but decreased blastocyst survival in previous experiments. Gilts received daily i.m. injections of 10 mg of DHT from day 13 (day 0 = onset of estrus) to day 18 (experiment 1), or from day 13 to 16 (experiment 2) of the estrous cycle. Gilts that served as controls received vehicle. The ovaries and a portion of uterine horn were surgically removed 24 h after the last treatment. Administration of DHT from day 13 to 18 of the estrous cycle decreased uterine wet weight (tendency, P = 0.10), and the relative amounts (ratios to ribosomal protein L19) of endometrial mRNA for the estrogen-responsive gene complement component C3. Gilts receiving DHT had greater amounts of ERbeta mRNA in the endometrium than those treated with vehicle in both experiments, but DHT did not alter the overall amounts of endometrial ERalpha mRNA. Immunohistochemical (IHC) analysis demonstrated that DHT did not alter the relative amounts of ERalpha in the myometrium, glandular and luminal epithelia and endometrial subepithelial stroma. In the ovary, amounts of ERalpha and ERbeta mRNAs in surface walls of follicles > or =6 mm in diameter were not altered by DHT treatments, however, DHT treatment from day 13 to 16 decreased the amounts of immunoreactive ERalpha in the theca interna at the surface walls of day 17 follicles (experiment 2). The amounts of immunoreactive ERalpha were greater in the granulosa than in the theca interna, and within cell type, the amounts of ERalpha were greater at the surface than at the basal region of the follicles, with the exception of the theca interna in follicles evaluated on day 19 (experiment 1). Treatment of gilts with DHT during the follicular phase of the estrous cycle increased ERbeta mRNA in the endometrium and influenced the amounts of immunoreactive ERalpha in ovarian follicles in a cell type-, day of development- and region-specific manner.
进行实验以评估在发情周期卵泡期用5α - 双氢睾酮（DHT）处理的后备母猪子宫和卵泡中雌激素受体（ER）α和ERβ基因的表达。在先前的实验中，这种DHT处理提高了排卵率，但降低了囊胚存活率。后备母猪在发情周期的第13天（第0天 = 发情开始）至第18天（实验1），或从第13天至第16天（实验2）每天肌肉注射10 mg DHT。作为对照的后备母猪注射赋形剂。在最后一次处理后24小时手术切除卵巢和一部分子宫角。在发情周期的第13天至第18天给予DHT可降低子宫湿重（趋势，P = 0.10），以及雌激素反应基因补体成分C3的子宫内膜mRNA的相对量（与核糖体蛋白L19的比率）。在两个实验中，接受DHT的后备母猪子宫内膜中ERβ mRNA的量均高于接受赋形剂处理的母猪，但DHT并未改变子宫内膜ERα mRNA的总量。免疫组织化学（IHC）分析表明，DHT并未改变子宫肌层、腺上皮和腔上皮以及子宫内膜上皮下基质中ERα的相对量。在卵巢中，直径≥6 mm卵泡表面壁中ERα和ERβ mRNA的量未因DHT处理而改变，然而，在实验2中，从第13天至第16天给予DHT可降低第17天卵泡表面壁内膜中免疫反应性ERα的量。颗粒层中免疫反应性ERα的量高于内膜，并且在细胞类型内，除了在实验1中评估的第19天卵泡的内膜外，卵泡表面的ERα量高于基部区域。在发情周期卵泡期用DHT处理后备母猪会增加子宫内膜中ERβ mRNA的量，并以细胞类型、发育天数和区域特异性方式影响卵巢卵泡中免疫反应性ERα的量。
