State Key Laboratory of Animal Nutrition, College of Animal Science & Technology, China Agricultural University, 2 Yuanmingyuan West Road, Beijing, 100193, PR China.
Poult Sci. 2019 Dec 1;98(12):7022-7029. doi: 10.3382/ps/pez367.
Genistein (GEN), a type of soy isoflavones, is similar to estrogen structurally and functionally. The effects of dietary gen on the reproductive performance and bone status of breeder hens were investigated. A total pf 720 laying broiler breeder (LBB) hens were randomly allocated into 3 groups with supplemental dietary GEN doses (0, 40, 400 mg/kg). Each treatment has 8 replicates of 30 birds. The results indicated that supplemental GEN significantly improved the egg production and eggshell strength of LBB hens. Dietary GEN was deposited into the egg yolk, which decreased malonaldehyde in the follicle and egg yolk. The levels of vitellogenin (VTG), progesterone, and follicle-stimulating hormone in the serum of GEN-treated groups were elevated compared with the control group. Furthermore, GEN treatment downregulated the mRNA expression of insulin-like growth factor binding protein in the fallopian tube, whereas 40 mg/kg GEN treatment upregulated estrogen receptor α expression. Both the mRNA expression of VTG-II in the liver and mRNA expression of amphiregulin in the fallopian tube were upregulated after 40 and 400 mg/kg GEN treatment. In the 400 mg/kg GEN-treated group, the levels of calcitonin and alkaline phosphatase in the serum were increased compared with the control group, which was consistent with the increased levels of calcium and phosphorus in the tibia. Supplemental GEN (400 mg/kg) improved the tibia strength of LBB hens, whereas 40 mg/kg GEN had better effects on laying performance. In summary, dietary GEN could improve the egg production and quality, as well as the bone status of LBB hens during the late egg-laying period.
染料木黄酮（GEN）是一种与雌激素在结构和功能上相似的大豆异黄酮。本研究旨在探讨日粮 GEN 对种母鸡繁殖性能和骨骼状况的影响。将 720 只产蛋期肉种鸡母鸡随机分为 3 组，每组 8 个重复，每个重复 30 只鸡。结果表明，日粮 GEN 显著提高了产蛋鸡的产蛋率和蛋壳强度。GEN 沉积在蛋黄中，降低了卵泡和蛋黄中的丙二醛含量。与对照组相比，GEN 处理组血清中卵黄蛋白原（VTG）、孕酮和促卵泡激素水平升高。此外，GEN 处理下调了输卵管中胰岛素样生长因子结合蛋白的 mRNA 表达，而 40mg/kg GEN 处理则上调了雌激素受体α的表达。40 和 400mg/kg GEN 处理后，肝脏中 VTG-II 的 mRNA 表达和输卵管中 Amphiregulin 的 mRNA 表达均上调。400mg/kg GEN 处理组血清降钙素和碱性磷酸酶水平较对照组升高，与胫骨钙、磷水平升高一致。日粮 GEN（400mg/kg）可提高产蛋后期肉种母鸡的胫骨强度，而 40mg/kg GEN 对产蛋性能的改善效果更好。综上所述，日粮 GEN 可改善产蛋后期肉种母鸡的产蛋性能和蛋品质，以及骨骼状况。
