Aravin Alexei, Tuschl Thomas
Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY 10021, USA.
FEBS Lett. 2005 Oct 31;579(26):5830-40. doi: 10.1016/j.febslet.2005.08.009. Epub 2005 Aug 18.
Double-stranded RNA (dsRNA) is a potent trigger of sequence-specific gene silencing mechanisms known as RNA silencing or RNA interference. The recognition of the target sequences is mediated by ribonucleoprotein complexes that contain 21- to 28-nucleotide (nt) guide RNAs derived from processing of the trigger dsRNA. Here, we review the experimental and bioinformatic approaches that were used to identify and characterize these small RNAs isolated from cells and tissues. The identification and characterization of small RNAs and their expression patterns is important for elucidating gene regulatory networks.
双链RNA（dsRNA）是序列特异性基因沉默机制（称为RNA沉默或RNA干扰）的有效触发因素。靶序列的识别由核糖核蛋白复合体介导，该复合体包含源自触发dsRNA加工的21至28个核苷酸（nt）的引导RNA。在这里，我们综述了用于鉴定和表征从细胞和组织中分离出的这些小RNA的实验和生物信息学方法。小RNA的鉴定、表征及其表达模式对于阐明基因调控网络很重要。
