Futenma A, Yamada H, Kitano M, Miyai H, Fukatsu A, Kato K
First Department of Internal Medicine, Aichi Medical University, Japan.
Nihon Jinzo Gakkai Shi. 1993 Apr;35(4):371-6.
To evaluate the significance of Cu,Zn-superoxide dismutase (SOD) in chronic renal disease, we evaluated the plasma SOD activity and analyzed the plasma Cu,Zn-SOD isomers employing gel column chromatography. The plasma SOD activity was determined as the biological activity using the nitrite method and the Cu,Zn-SOD concentration was assayed from the immunological activity using enzyme-linked immunosorbent assay (ELISA). The subjects comprised 185 patients with chronic glomerular disease and 20 hemodialysis patients. Plasma from 170 healthy persons was employed as a control. Both the plasma biological activity and plasma level of Cu,Zn-SOD determined by ELISA were elevated in patients with chronic glomerular disease. In hemodialysis patients, a marked increase in Cu,Zn-SOD level (ELISA) was noted in comparison with the increase in SOD biological activities. Gel column chromatography demonstrated a marked increase in Cu,Zn-SOD monomer which was enzymatically inactive. From these results, we conclude that a marked elevation of the plasma level of Cu,Zn-SOD in hemodialysis patients was caused by an increase in the enzymatically inactive Cu,Zn-SOD monomer.
为评估铜锌超氧化物歧化酶（SOD）在慢性肾病中的意义，我们测定了血浆SOD活性，并采用凝胶柱色谱法分析血浆铜锌SOD异构体。血浆SOD活性采用亚硝酸盐法测定生物学活性，铜锌SOD浓度采用酶联免疫吸附测定法（ELISA）从免疫活性进行检测。研究对象包括185例慢性肾小球疾病患者和20例血液透析患者。选取170名健康人的血浆作为对照。慢性肾小球疾病患者血浆的生物学活性以及ELISA测定的铜锌SOD水平均升高。血液透析患者中，与SOD生物学活性的升高相比，铜锌SOD水平（ELISA法）显著升高。凝胶柱色谱显示酶活性缺失的铜锌SOD单体显著增加。根据这些结果，我们得出结论，血液透析患者血浆铜锌SOD水平显著升高是由酶活性缺失的铜锌SOD单体增加所致。
