Blazek M, Naser W, Bayer-Beck J, Lakomek H J, Werle E, Fiehn W
Zentrallabor der Medizinischen Klinik und Poliklinik Heidelberg.
Z Rheumatol. 1992 Mar-Apr;51(2):87-93.
Antinuclear antibodies are of major importance in the diagnosis of inflammatory rheumatic diseases. Sm and nRNP antibodies can be found in sera from patients with systemic lupus erythematosus and mixed connective tissue disease. Usually, these antibodies have been detected with one of the following methods: Ouchterlony immunodiffusion, passive hemagglutination or counterimmunoelectrophoresis (CIE). In this work results obtained by Ouchterlony and CIE techniques were compared with those obtained by ELISA. Purified proteins from cellular extracts (HeLa) were used as antigens for Sm- and nRNP-ELISA: D polypeptide for Sm-ELISA and the 68 kD, A, C, B,B' and D polypeptides for nRNP-ELISA. Compared with the other two techniques, ELISA was less time consuming and showed greater sensitivity. Quantitative titration proved to be of advantage in monitoring the course of the diseases mentioned above.
抗核抗体在炎性风湿性疾病的诊断中具有重要意义。Sm抗体和nRNP抗体可在系统性红斑狼疮和混合性结缔组织病患者的血清中发现。通常，这些抗体是通过以下方法之一检测到的：免疫双扩散法、被动血凝法或对流免疫电泳法（CIE）。在这项研究中，将免疫双扩散法和对流免疫电泳法的检测结果与酶联免疫吸附测定法（ELISA）的结果进行了比较。来自细胞提取物（HeLa）的纯化蛋白用作Sm-ELISA和nRNP-ELISA的抗原：用于Sm-ELISA的D多肽以及用于nRNP-ELISA的68 kD、A、C、B、B'和D多肽。与其他两种技术相比，ELISA耗时更少且灵敏度更高。定量滴定被证明在监测上述疾病的病程方面具有优势。
