Reichlin M, Harley J B
Trans Assoc Am Physicians. 1986;99:161-71.
Three hundred five consecutive patients with systemic lupus erythematosus (SLE) have been evaluated for antibodies to the RNA protein particles, Sm, nRNP, Ro/SSA, and La/SSB. Precipitating antibodies were found in 224 (73.1%). With the use of ELISA methodology with affinity-purified antigens, 76 sera from precipitin-negative patients were assayed and 35 had elevated levels of one or more of the autoantibodies. Of these, 27 (36%) had elevated anti-nRNP and 17 (22%) had elevated anti-Ro/SSA. Six of the seven patients with anti-La/SSB (18%) also had anti-Ro/SSA paralleling the close association of these two antibodies noted by precipitin analysis in which 38 of 39 patients with anti-La/SSB precipitins also have anti-Ro/SSA precipitins. Thus, 258 of the 305 SLE sera (84.6%) have elevated anti-RNA protein levels. This represents a minimum estimate of the frequency of these antibodies since longitudinal study of individual patients shows that anti-RNA protein levels may vary from normal to 1000 times over background at different times. Quantitative studies over time in two patients with both anti-Sm and anti-nRNP show that anti-nRNP increased 10- to 100-fold while anti-Sm changed little. These quantitative changes may relate to clinical phenomena.
对305例系统性红斑狼疮（SLE）患者连续进行了RNA蛋白颗粒、Sm、nRNP、Ro/SSA和La/SSB抗体检测。发现224例（73.1%）有沉淀抗体。采用亲和纯化抗原的ELISA方法，对76例沉淀阴性患者的血清进行检测，其中35例一种或多种自身抗体水平升高。其中，27例（36%）抗nRNP升高，17例（22%）抗Ro/SSA升高。7例抗La/SSB患者中有6例（18%）也有抗Ro/SSA，这与沉淀分析中发现的这两种抗体的密切关联一致，在39例有抗La/SSB沉淀的患者中，38例也有抗Ro/SSA沉淀。因此，305例SLE血清中有258例（84.6%）抗RNA蛋白水平升高。这只是这些抗体频率的最低估计值，因为对个体患者的纵向研究表明，抗RNA蛋白水平在不同时间可能从正常水平变化到高于背景值1000倍。对两名同时有抗Sm和抗nRNP的患者进行的长期定量研究表明，抗nRNP增加了10到100倍，而抗Sm变化不大。这些定量变化可能与临床现象有关。
