Rosso A, Ferrarotti S, Miranda M V, Krymkiewicz N, Nudel B C, Cascone O
Departamento de Ciencias Básicas, Universidad Nacional de Luján, Rutas 5 y 7, 6700, Luján, Buenos Aires, Argentina.
Biotechnol Lett. 2005 Aug;27(16):1171-5. doi: 10.1007/s10529-005-8654-6.
Two rapid and easy-to-scale-up methods for the purification of cyclodextrin glycosyltransferase (CGTase) from Bacillus circulans were developed: affinity precipitation with starch and aqueous two-phase partition. The first method, optimised by a factorial design, gave an 80% CGTase adsorption at 11% starch and 1.6% ammonium sulphate, and a 65% recovery after elution with 10 mM alpha-cyclodextrin. The purification factor was 17. Aqueous two-phase partition yielded a 72% CGTase recovery in a two-step procedure; CGTase was obtained in the bottom phase with a purification factor of 37.
