Ferrarotti S A, Rosso A M, Maréchal M A, Krymkiewicz N, Maréchal L R
Universidad Nacional de Luján, Departamento de Ciencias Básicas, Argentina.
Cell Mol Biol (Noisy-le-grand). 1996 Jul;42(5):653-7.
From wild type inoculum of Bacillus circulans DF 9 which produce cyclomaltodextrin-glucanotransferase (EC 2.4.1.19, CGTase), two different kinds of colonies were isolated, which correspond to the classical S-R variation. From the culture medium of both colonies grown together a CGTase was purified about 50 fold with a yield of 54% in two steps. From pure R-cell culture the enzyme was purified by about 38 folds with a yield of 79% in only one step, showing a complete homogeneity as judged by a native PAGE analysis.
