Bothe Gerald W M, Bolivar Valerie J, Vedder Michelle J, Geistfeld James G
Taconic Farms, Germantown, New York 12526, USA.
Comp Med. 2005 Aug;55(4):326-34.
We compared the behavior of 14 inbred mouse strains and an F1 hybrid commonly used in transgenic and knockout production. These strains were 129P3/J, 129S1/SvImJ, 129S6/SvEvTac, 129T2/SvEmsJ, 129X1/SvJ (formerly 129/J, 129/Sv-p+Tyr+Kitl+/J, 129/SvEvTac, 129SvEmsJ, and 129/SvJ, respectively), A/JCrTac, BALB/cAnNTac, C3H/HeNTac, C57BL/6J, C57BL/6NTac, DBA/2NTac, FVB/NTac, NOD/MrkTac, SJL/JCrNTac, and the hybrid B6129S6F1Tac. Performance in three behavioral tests (rotorod, open-field activity-habituation, and contextual and cued fear conditioning) was determined. On the rotorod assay, SJL/JCrNTac mice had the shortest latencies to fall on the first day of testing, and DBA/2NTac mice showed impaired motor learning. Open-field behavior was analyzed using the parameters total distance, center distance, velocity, and vertical activity. 129T2/EvEmsJ and A/JCrTac were least active in the open field, whereas NOD/MrkTac mice were most active. Contrary to earlier studies, we found that all strains habituated to the open field in at least one of these parameters. In contextual and cued fear conditioning, all strains displayed activity suppression. However, FVB/NTac mice reacted less strongly to both context and cue than did most of the other strains. There were no significant behavioral differences between C57BL/6J and C57BL/6NTac, except for higher open-field activity in C57BL/6J female mice. These findings illustrate the importance of the appropriate selection of background strain for transgenic, gene targeting, or drug research.
我们比较了14种近交系小鼠品系以及一种常用于转基因和基因敲除实验的F1杂交小鼠的行为表现。这些品系包括129P3/J、129S1/SvImJ、129S6/SvEvTac、129T2/SvEmsJ、129X1/SvJ（分别为之前的129/J、129/Sv-p+Tyr+Kitl+/J、129/SvEvTac、129SvEmsJ和129/SvJ）、A/JCrTac、BALB/cAnNTac、C3H/HeNTac、C57BL/6J、C57BL/6NTac、DBA/2NTac、FVB/NTac、NOD/MrkTac、SJL/JCrNTac以及杂交种B6129S6F1Tac。测定了它们在三项行为测试（转棒试验、旷场活动习惯化以及情境性和线索性恐惧条件反射）中的表现。在转棒试验中，SJL/JCrNTac小鼠在测试第一天掉落所需的潜伏期最短，而DBA/2NTac小鼠表现出运动学习障碍。使用总距离、中心距离、速度和垂直活动等参数分析旷场行为。129T2/EvEmsJ和A/JCrTac在旷场中活动最少，而NOD/MrkTac小鼠活动最为活跃。与早期研究相反，我们发现所有品系在这些参数中的至少一项上对旷场产生了习惯化。在情境性和线索性恐惧条件反射中，所有品系均表现出活动抑制。然而，FVB/NTac小鼠对情境和线索的反应比大多数其他品系都要弱。除了C57BL/6J雌性小鼠在旷场中的活动较高外，C57BL/6J和C57BL/6NTac之间没有显著的行为差异。这些发现说明了为转基因、基因靶向或药物研究适当选择背景品系的重要性。
