Halm D R, Rick R
Department of Physiology, Ohio State University, Columbus 43210.
Am J Physiol. 1992 Jun;262(6 Pt 1):C1392-402. doi: 10.1152/ajpcell.1992.262.6.C1392.
Electron microprobe analysis of quick-frozen distal colonic epithelium from guinea pig was used to locate the cells responding to secretory stimuli. Concentrations of Na, K, and Cl were similar for cells of surface and crypt in the unstimulated state, 8, 149, and 46 mmol/kg wet weight, respectively. Stimulation of either K and Cl secretion with prostaglandin E2 or K secretion alone with epinephrine increased Na to approximately 12 mmol/kg wet weight in crypt cells but not in surface cells or cells in the crypt neck. This result supports the location of ion secretory cells in the lower two-thirds of the crypt. In the vacuoles of crypt columnar cells, stimulation of KCl secretion decreased K, S, Mg, and Ca and increased Na and Cl, indicative of the concomitant release of vacuole contents. Mucin granules in crypt goblet cells contained more S and Mg than granules in surface goblet cells. These findings support the concept of differentiation in ion and macromolecular secretory function along the axis from crypt to surface epithelium.
利用电子微探针分析豚鼠快速冷冻的远端结肠上皮，以定位对分泌刺激有反应的细胞。在未受刺激状态下，表面细胞和隐窝细胞中的钠、钾和氯浓度相似，分别为8、149和46 mmol/kg湿重。用前列腺素E2刺激钾和氯分泌或仅用肾上腺素刺激钾分泌，会使隐窝细胞中的钠增加到约12 mmol/kg湿重，但表面细胞或隐窝颈部细胞中的钠不会增加。这一结果支持离子分泌细胞位于隐窝下三分之二处的观点。在隐窝柱状细胞的液泡中，刺激氯化钾分泌会使钾、硫、镁和钙减少，钠和氯增加，表明液泡内容物同时释放。隐窝杯状细胞中的黏液颗粒比表面杯状细胞中的颗粒含有更多的硫和镁。这些发现支持了沿隐窝到表面上皮轴在离子和大分子分泌功能方面存在分化的概念。
