Wierzchowski J, Holmquist B, Vallee B L
Center for Biochemical and Biophysical Sciences, Harvard Medical School, Boston, Massachusetts 02115.
Anal Chem. 1992 Jan 15;64(2):181-6. doi: 10.1021/ac00026a017.
Both class I and class II alcohol dehydrogenase (ADH) activities are present in human serum. The contribution of each class can be measured using two class-specific, fluorogenic substrates, 4-methoxy-1-naphthaldehyde and 6-methoxy-2-naphthaldehyde. The former is highly selective for class I isozymes, especially those containing alpha or gamma subunits, whereas class II (pi) ADH preferentially reduces the latter. Selective inhibition of class I ADH by 4-methylpyrazole further increases the specificity. Specificity, accuracy, and precision of the assay for serum measurements have been determined. The activity of class I ADH in normal human serum is below the limit of detection of this method, i.e., less than 1.0 nM/min. The activity of class II ADH in normal individuals is 15 +/- 5 nM/min. In some patients values as high as 2100 nM/min are observed for class I, but in all instances, the amount of class II found was higher than that of class I ADH.
I类和II类乙醇脱氢酶（ADH）活性在人血清中均有存在。每一类的贡献可以使用两种类特异性的荧光底物4-甲氧基-1-萘甲醛和6-甲氧基-2-萘甲醛来测量。前者对I类同工酶具有高度选择性，尤其是那些含有α或γ亚基的同工酶，而II类（π）ADH则优先还原后者。4-甲基吡唑对I类ADH的选择性抑制进一步提高了特异性。已经确定了用于血清测量的该检测方法的特异性、准确性和精密度。正常人类血清中I类ADH的活性低于该方法的检测限，即低于1.0 nM/分钟。正常个体中II类ADH的活性为15±5 nM/分钟。在一些患者中，观察到I类ADH的值高达2100 nM/分钟，但在所有情况下，发现的II类ADH的量均高于I类ADH。
