Simpson J L, Wood L G, Gibson P G
School of Medical Practice and Population Health, The University of Newcastle, Callaghan NSW, Australia.
Clin Exp Allergy. 2005 Sep;35(9):1180-5. doi: 10.1111/j.1365-2222.2005.02327.x.
Airway inflammation is assessed to monitor progression, control and treatment of asthma. The collection of exhaled breath condensate (EBC) provides a non-invasive alternative to induced sputum samples for the monitoring of airway inflammation. Both samples can be confounded by salivary contamination. The aim of this study was to compare the levels of inflammatory mediators in samples of EBC, induced sputum and saliva samples from subjects with asthma.
EBC, saliva and induced sputum samples were collected from subjects with asthma (n=10). Total protein, IL-8, 8-isoprostane and surfactant protein A (SPA) were assessed in each sample.
Total protein, IL-8, 8-isoprostane and SPA were detected in all sputum samples. Only total protein and SPA were consistently measured in EBC, with levels at least 100-fold lower than those measured in induced sputum. In saliva, total protein, SPA and 8-isoprostane were detected in all samples, with IL-8 detected in 60% of samples.
Induced sputum is a reliable technique that can be used to assess markers of airway inflammation. While EBC is a simple and inexpensive technique to collect lower airway secretions, the detection of inflammatory mediators is variable, and further work is required to validate this technique to assess inflammatory mediators.
评估气道炎症以监测哮喘的进展、控制和治疗情况。收集呼出气冷凝液（EBC）为监测气道炎症提供了一种非侵入性替代诱导痰样本的方法。这两种样本都可能受到唾液污染的影响。本研究的目的是比较哮喘患者的EBC样本、诱导痰样本和唾液样本中炎症介质的水平。
从哮喘患者（n = 10）中收集EBC、唾液和诱导痰样本。对每个样本评估总蛋白、白细胞介素-8（IL-8）、8-异前列腺素和表面活性蛋白A（SPA）。
在所有痰样本中均检测到总蛋白、IL-8、8-异前列腺素和SPA。在EBC中仅能持续检测到总蛋白和SPA，其水平比诱导痰中检测到的水平至少低100倍。在唾液中，所有样本均检测到总蛋白、SPA和8-异前列腺素，60%的样本检测到IL-8。
诱导痰是一种可用于评估气道炎症标志物的可靠技术。虽然EBC是一种收集下呼吸道分泌物的简单且廉价的技术，但炎症介质的检测结果存在差异，需要进一步开展工作以验证该技术用于评估炎症介质的有效性。
