Mazur Witold, Stark Harri, Sovijärvi Anssi, Myllärniemi Marjukka, Kinnula Vuokko L
Biomedicum Helsinki and Department of Internal Medicine, Division of Pulmonary Medicine, University of Helsinki and Helsinki University Central Hospital, FI-00014 Helsinki, Finland.
Respiration. 2009;78(2):209-16. doi: 10.1159/000206010. Epub 2009 Mar 2.
Markers of airway inflammation and oxidative stress have been mainly investigated in moderate/severe chronic obstructive pulmonary disease (COPD) or during its exacerbation. They have not been compared in noninvasive specimens such as exhaled breath condensate (EBC) and induced sputum in healthy nonsymptomatic smokers or in those who have symptoms and are at risk for COPD development.
To compare the relative proportions of 2 potential COPD biomarkers, 8-isoprostane and interleukin- 8 (IL-8) in the induced sputum and EBC sampled from the same subjects: nonsmokers (n = 14), healthy smokers (n = 17) and symptomatic smokers (n = 9) who are considered to be at risk for COPD. COPD patients with acute exacerbation (n = 10) were employed as positive controls.
The levels of the aforementioned biomarkers in induced sputum and EBC were investigated using commercial biochemical techniques.
In induced sputum, the levels of 8-isoprostane and IL-8 were at least 10-fold higher compared to EBC levels in all groups. Healthy nonsmokers had the lowest levels, and patients with exacerbation of COPD the highest levels of 8-isoprostane in induced sputum and EBC. The same observation held true for IL-8 in induced sputum. Inverse correlations with lung function parameters were observed for both mediators.
The levels of both potential markers were clearly higher in the induced sputum than in EBC. The results point to an advantage of induced sputum over EBC for assessing the degree of airway oxidative stress and inflammation in smokers with a potential risk for COPD development.
气道炎症和氧化应激标志物主要在中度/重度慢性阻塞性肺疾病（COPD）或其急性加重期进行研究。尚未在无创样本（如呼出气冷凝液（EBC））以及诱导痰中，对健康无症状吸烟者或有症状且有患COPD风险者的这些标志物进行比较。
比较从同一组受试者采集的诱导痰和EBC中两种潜在的COPD生物标志物——8-异前列腺素和白细胞介素-8（IL-8）的相对比例：非吸烟者（n = 14）、健康吸烟者（n = 17）和有症状吸烟者（n = 9），这些有症状吸烟者被认为有患COPD的风险。将COPD急性加重期患者（n = 10）作为阳性对照。
采用商业生化技术研究诱导痰和EBC中上述生物标志物的水平。
在诱导痰中，所有组的8-异前列腺素和IL-8水平均比EBC水平至少高10倍。健康非吸烟者的水平最低，COPD急性加重期患者的诱导痰和EBC中8-异前列腺素水平最高。诱导痰中IL-8的情况也相同。两种介质均与肺功能参数呈负相关。
两种潜在标志物在诱导痰中的水平明显高于EBC。结果表明，对于评估有患COPD潜在风险的吸烟者的气道氧化应激和炎症程度，诱导痰优于EBC。
