Lee S S, Kang C
Department of Life Science, Korea Advanced Institute of Science and Technology, Taejon.
Biochem Int. 1992 Feb;26(1):1-5.
The phage T7 and SP6 RNA polymerase-promoter systems are very similar in many characteristics, but maintains stringent specificity for each. In order to identify the base pair element that distinguishes between T7 and SP6 promoters, the base pairs at -12, -10, -9, and -8 of the T7 promoter consensus sequence were changed singly and multiply to the SP6 promoter-specific base pairs, and assayed for T7 and SP6 promoter activities. The results indicate that the primary discrimination element is the base pairs at -8 and -9. The two-base-pair substitution alone in T7 promoter by SP6-specific base pairs is sufficient to make the T7 variant be a SP6 promoter, abolishing T7 promoter activity.
噬菌体T7和SP6 RNA聚合酶-启动子系统在许多特性上非常相似，但对各自的启动子保持严格的特异性。为了鉴定区分T7和SP6启动子的碱基对元件，将T7启动子共有序列中-12、-10、-9和-8位的碱基对逐个或多个地替换为SP6启动子特异性碱基对，并检测其T7和SP6启动子活性。结果表明，主要的鉴别元件是-8和-9位的碱基对。仅将T7启动子中的两个碱基对替换为SP6特异性碱基对就足以使T7变体成为SP6启动子，从而消除T7启动子活性。
