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[DNA的稳定性和局部协同性分析：关于大肠杆菌RNA聚合酶启动噬菌体T7基因A3转录的分子机制的提议]

[Analysis of the stability and local cooperativity of DNA: proposal of a molecular mechanism for the initiation of the transcription of gene A3 of bacteriophage T7 by the RNA polymerase from E. coli].

作者信息

Ehrlich R, Marín M, Gabarró-Arpa J, Rodier F, Schmitt B, Reiss C

出版信息

C R Seances Acad Sci III. 1981 Jan 12;292(2):177-80.

PMID:6783340
Abstract

RNA polymerase (RNAP) complexed to the A3 promoter of bacteriophage T7 is known to unwind a DNA segment located downstream of the Pribnow box. This finding can be accounted for if it is assumed that the subunit sigma of RNAP unstabilizes three GC base pairs located just upstream of the transcription start. As a consequence, the rate of promoter utilisation might be related to the relative stability of the DNA between the "Pribnow box" and the transcription start.

摘要

已知与噬菌体T7的A3启动子复合的RNA聚合酶（RNAP）会解开位于普里布诺框下游的一段DNA。如果假设RNAP的σ亚基使位于转录起始点上游的三个GC碱基对不稳定，那么这一发现就可以得到解释。因此，启动子的利用速率可能与“普里布诺框”和转录起始点之间DNA的相对稳定性有关。

相似文献

1
[Analysis of the stability and local cooperativity of DNA: proposal of a molecular mechanism for the initiation of the transcription of gene A3 of bacteriophage T7 by the RNA polymerase from E. coli].[DNA的稳定性和局部协同性分析：关于大肠杆菌RNA聚合酶启动噬菌体T7基因A3转录的分子机制的提议]
C R Seances Acad Sci III. 1981 Jan 12;292(2):177-80.
2
[Properties of cloned promoters].[克隆启动子的特性]
Mol Biol (Mosk). 1984 Jan-Feb;18(1):130-9.
3
[Analysis of the stability and local cooperativity of DNA : elements permitting the recognition of promoters by DNA-dependent RNA polymerase].[DNA的稳定性和局部协同性分析：允许依赖DNA的RNA聚合酶识别启动子的元件]
C R Seances Acad Sci III. 1981 Jan 5;292(1):5-8.
4
Promoter recognition and transcription initiation in E. coli.大肠杆菌中的启动子识别与转录起始
Folia Biol (Praha). 1984;30 Spec No:105-18.
5
A two-base-pair substitution in T7 promoter by SP6 promoter-specific base pairs alone abolishes T7 promoter activity but reveals SP6 promoter activity.仅通过用SP6启动子特异性碱基对替换T7启动子中的两个碱基对，就可消除T7启动子活性，但会显现出SP6启动子活性。
Biochem Int. 1992 Feb;26(1):1-5.
6
Aromatic amino acids in region 2.3 of Escherichia coli sigma 70 participate collectively in the formation of an RNA polymerase-promoter open complex.大肠杆菌σ70因子2.3区域中的芳香族氨基酸共同参与RNA聚合酶-启动子开放复合物的形成。
J Mol Biol. 2000 Jun 23;299(5):1217-30. doi: 10.1006/jmbi.2000.3808.
7
[Role of the beta-subunits of Escherichia coli RNA-polymerase in the regulation of gene activity].[大肠杆菌RNA聚合酶β亚基在基因活性调控中的作用]
Mol Biol (Mosk). 1980 Jul-Aug;14(4):759-65.
8
[Coupling of bacteriophage T7 DNA penetration with its transcription, during infection].[感染期间噬菌体T7 DNA穿透与其转录的偶联]
Mol Biol (Mosk). 1983 Sep-Oct;17(5):1048-59.
9
An Escherichia coli RNA polymerase defective in transcription due to its overproduction of abortive initiation products.一种由于流产起始产物过量产生而在转录方面存在缺陷的大肠杆菌RNA聚合酶。
J Mol Biol. 1994 Feb 11;236(1):72-80. doi: 10.1006/jmbi.1994.1119.
10
Beta subunit residues 186-433 and 436-445 are commonly used by Esigma54 and Esigma70 RNA polymerase for open promoter complex formation.β亚基的186 - 433位残基以及436 - 445位残基通常被埃希氏菌σ54和埃希氏菌σ70 RNA聚合酶用于开放启动子复合物的形成。
J Mol Biol. 2002 Jun 21;319(5):1067-83. doi: 10.1016/S0022-2836(02)00330-3.

引用本文的文献

1
Physical characteristics in eucaryotic promoters.真核生物启动子中的物理特征。
Nucleic Acids Res. 1983 Jul 11;11(13):4521-40. doi: 10.1093/nar/11.13.4521.
2
DNA sequences specifying the transcription of the streptococcal kanamycin resistance gene in Escherichia coli and Bacillus subtilis.指定大肠杆菌和枯草芽孢杆菌中链球菌卡那霉素抗性基因转录的DNA序列。
Mol Gen Genet. 1985;198(2):348-52. doi: 10.1007/BF00383017.
3
Nucleotide sequence of the kanamycin resistance determinant of the pneumococcal transposon Tn1545: evolutionary relationships and transcriptional analysis of aphA-3 genes.
肺炎球菌转座子Tn1545卡那霉素抗性决定簇的核苷酸序列：aphA-3基因的进化关系及转录分析
Mol Gen Genet. 1987 May;207(2-3):509-13. doi: 10.1007/BF00331623.