Martínez-Checa Fernando, Béjar Victoria, Martínez-Cánovas M José, Llamas Inmaculada, Quesada Emilia
Microbial Exopolysaccharide Research Group, Department of Microbiology, Faculty of Pharmacy, Campus Universitario de Cartuja s/n, University of Granada, 18071 Granada, Spain.
Int J Syst Evol Microbiol. 2005 Sep;55(Pt 5):2007-2011. doi: 10.1099/ijs.0.63676-0.
Halomonas almeriensis sp. nov. is a Gram-negative non-motile rod that was isolated from a saltern in the Cabo de Gata-Níjar wildlife reserve in Almería, south-east Spain. It is moderately halophilic, capable of growth at concentrations of 5-25% w/v sea-salt mixture, the optimum being 7.5% w/v. It is chemo-organotrophic and strictly aerobic, produces catalase but not oxidase, does not produce acid from any sugar and does not synthesize hydrolytic enzymes. The most notable difference between this micro-organism and other Halomonas species is that it is very fastidious in its use of a carbon source. It forms mucoid colonies due to the production of an exopolysaccharide. Its G+C content is 63.5 mol%. A comparison of 16S rRNA gene sequences confirmed its relationship to Halomonas species. The most closely related species is Halomonas halmophila with 95.8% similarity between their 16S rRNA gene sequences. DNA-DNA hybridization with H. halmophila is 10.1%. Its major fatty acids are 18:1omega7c, 16:0, 16:1omega7c/15:0 iso 2-OH, 12:0 3-OH, 12:0, 11-methyl 18:1omega7c and 10:0. The proposed name is Halomonas almeriensis sp. nov., with strain M8(T) (=CECT 7050(T)=LMG 22904(T)) as the type strain.
阿尔梅里亚盐单胞菌新种是一种革兰氏阴性、无运动性的杆菌，从西班牙东南部阿尔梅里亚的加塔角-尼哈尔野生动物保护区的一个盐场中分离得到。它是中度嗜盐菌，能够在5-25%（w/v）的海盐混合物浓度下生长，最适浓度为7.5%（w/v）。它是化能有机营养型且严格需氧，产生过氧化氢酶但不产生氧化酶，不能从任何糖类产酸，也不合成水解酶。这种微生物与其他盐单胞菌属物种最显著的区别在于其对碳源的利用非常挑剔。由于产生胞外多糖，它形成黏液状菌落。其G+C含量为63.5 mol%。16S rRNA基因序列比较证实了它与盐单胞菌属物种的关系。最密切相关的物种是嗜盐盐单胞菌，其16S rRNA基因序列相似度为95.8%。与嗜盐盐单胞菌的DNA-DNA杂交率为10.1%。其主要脂肪酸为18:1ω7c、16:0、16:1ω7c/15:0异2-OH、12:0 3-OH、12:0、11-甲基18:1ω7c和10:0。提议的名称为阿尔梅里亚盐单胞菌新种，菌株M8(T)（=CECT 7050(T)=LMG 22904(T)）为模式菌株。
