Biotechnology and Applied Microbiology Research Group, Department of Biology, Faculty of Sciences, University Abdelmalek Essâadi, BP2121, 93002 Tetouan, Morocco.
Microbial Exopolysaccharide Research Group, Department of Microbiology, Faculty of Pharmacy, Cartuja Campus, University of Granada, 18071 Granada, Spain.
Int J Syst Evol Microbiol. 2011 Nov;61(Pt 11):2600-2605. doi: 10.1099/ijs.0.027268-0. Epub 2010 Dec 3.
A polyphasic taxonomic study was conducted on strain HK31(T), a moderately halophilic bacterium isolated from a solar saltern in Chefchaouen, Morocco. The strain was a Gram-reaction-negative, oxidase-positive rod, which was motile by means of peritrichous flagella. The strain required NaCl for growth and grew in salt concentrations (mixture of sea salts) of 0.5-20 % (w/v) (optimum 5-7.5 %, w/v), at 25-45 °C (optimum 32 °C) and at pH 5-10 (optimum pH 6-9). Strain HK31(T) did not produce acids from sugars and its metabolism was respiratory, using oxygen as terminal electron acceptor. The strain was positive for the accumulation of poly-β-hydroxyalkanoate granules and formed mucoid colonies due to the excretion of an exopolysaccharide. The DNA G+C content was 61.5 mol%. 16S rRNA gene sequence analysis indicated that it belonged to the genus Halomonas in the class Gammaproteobacteria. The most phylogenetically related species was Halomonas anticariensis, with which strain HK31(T) showed a 16S rRNA gene sequence similarity of 96.48 %. Its major fatty acids were C(18 : 1)ω7c, C(16 : 0), C(19 : 0) cyclo ω8c, C(16 : 1)ω7c/iso-C(15 : 0) 2-OH and C(12 : 0) 3-OH and the predominant respiratory lipoquinone was ubiquinone with nine isoprene units (Q-9). Based on the evidence provided in this study, strain HK31(T) (= CECT 7698(T) = LMG 25695(T)) represents a novel species of the genus Halomonas, for which the name Halomonas rifensis is proposed.
对从摩洛哥舍夫沙万的一个太阳能盐场分离到的中度嗜盐细菌 HK31(T) 菌株进行了多相分类学研究。该菌株为革兰氏反应阴性、氧化酶阳性的杆状菌，通过周生鞭毛运动。该菌株的生长需要 NaCl，在盐浓度（海水盐混合物）为 0.5-20%（w/v）（最佳为 5-7.5%，w/v）、25-45°C（最佳 32°C）和 pH 5-10（最佳 pH 6-9）的条件下生长。菌株 HK31(T) 不能从糖中产生酸，其代谢为呼吸作用，以氧作为末端电子受体。该菌株聚β-羟基烷酸颗粒积累阳性，并由于排泄胞外多糖而形成粘液状菌落。DNA G+C 含量为 61.5 mol%。16S rRNA 基因序列分析表明，它属于γ变形菌纲的盐单胞菌属。与菌株 HK31(T)亲缘关系最近的物种是抗卡利的盐单胞菌，其 16S rRNA 基因序列相似性为 96.48%。其主要脂肪酸为 C(18:1)ω7c、C(16:0)、C(19:0) 环 ω8c、C(16:1)ω7c/iso-C(15:0)2-OH 和 C(12:0)3-OH，主要呼吸类脂醌为九个异戊二烯单位的泛醌（Q-9）。根据本研究提供的证据，菌株 HK31(T)（= CECT 7698(T)=LMG 25695(T)）代表盐单胞菌属的一个新种，现提议将其命名为 rifensis 盐单胞菌。
