Direct microtiter plate sequencing of PCR-amplified M13 clones from plaques using dried reagents.
作者信息
Dale S J
机构信息
Amersham International plc, Little Chalfont, Bucks, UK.
出版信息
Biotechniques. 1992 Feb;12(2):194, 196-7.
PMID:1616708
Abstract
A method is described using dried reagents in microtiter plates for direct PCR and sequencing of M13 plaques. The method has advantages of reliability and high throughput that make it suitable for medium to large-scale sequencing projects.
摘要
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