Paltrinieri Saverio, Comazzi Stefano, Ceciliani Fabrizio, Prohaska Rainer, Bonfanti Ugo
Department of Veterinary Pathology, Hygiene and Public Health, Unit of Veterinary General Pathology and Parasitology, University of Milan Via Celoria 10, 20133, Milan, Italy.
Vet J. 2007 Jan;173(1):200-3. doi: 10.1016/j.tvjl.2005.07.009. Epub 2005 Sep 13.
Stomatocytosis resembles human overhydrated hereditary stomatocytosis (OHSt), a disease characterised by a reduced or absent stomatin expression. The objective of this report was to investigate the expression level of stomatin in erythrocytes from Standard Schnauzers with stomatocytosis. Routine haematology, intraerythrocytic Na(+)/K(+) concentration and stomatin expression were evaluated in blood from twelve Standard Schnauzers and from three controls. SDS-PAGE and Western blotting on isolated integral membrane proteins were used to investigate stomatin expression. Circulating stomatocytes, macrocytosis, anisocytosis, increased erythrocyte fragility and high intracellular sodium and potassium concentrations were found in 10/12 dogs from the same breeding line although stomatin levels were similar to those of controls. In spite of the clinico-pathological similarities between human and canine stomatocytosis, erythrocytes from affected dogs do not lack stomatin and the expression level of this protein cannot therefore be used to diagnose hereditary stomatocytosis in Standard Schnauzers.
口形红细胞增多症类似于人类的遗传性水过多性口形红细胞增多症（OHSt），这是一种以口形蛋白表达减少或缺失为特征的疾病。本报告的目的是研究患有口形红细胞增多症的标准雪纳瑞犬红细胞中口形蛋白的表达水平。对12只标准雪纳瑞犬和3只对照犬的血液进行了常规血液学检查、红细胞内钠/钾浓度测定以及口形蛋白表达评估。采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和对分离的整合膜蛋白进行蛋白质免疫印迹法来研究口形蛋白的表达。在来自同一繁育系的12只犬中的10只犬身上发现了循环口形红细胞、大红细胞症、红细胞大小不均、红细胞脆性增加以及细胞内高钠和高钾浓度，尽管口形蛋白水平与对照犬相似。尽管人类和犬类口形红细胞增多症在临床病理方面存在相似之处，但患病犬的红细胞并不缺乏口形蛋白，因此该蛋白的表达水平不能用于诊断标准雪纳瑞犬的遗传性口形红细胞增多症。
