7只相关标准雪纳瑞犬中的口形红细胞症

Stomatocytosis in 7 related Standard Schnauzers.

作者信息

Bonfanti Ugo, Comazzi Stefano, Paltrinieri Saverio, Bertazzolo Walter

机构信息

Clinica Veterinaria Gran Sasso, Milano, Italy.

出版信息

Vet Clin Pathol. 2004;33(4):234-9. doi: 10.1111/j.1939-165x.2004.tb00379.x.

DOI:10.1111/j.1939-165x.2004.tb00379.x

PMID:15570561

Abstract

BACKGROUND

Hereditary canine stomatocytosis has been described in purebred Alaskan Malamutes, Drentse Patrijshonds, and Miniature Schnauzers. In humans, hereditary stomatocytosis is a heterogeneous group of congenital disorders characterized by the presence of stomatocytes in blood, increased osmotic fragility, and frequently, hemolytic anemia.

OBJECTIVE

Our objective was to describe hematologic findings and RBC characteristics in 7 closely related Standard Schnauzers with stomatocytosis.

METHODS

The following parameters were measured using an automated analyzer: HCT, RBC, hemoglobin (Hb) concentration, MCV, MCH, MCHC, red cell distribution width (RDW), WBC, platelet count, mean platelet volume (MPV), thrombocrit (PCT), and platelet distribution width (PDW). Differential leukocyte count, platelet estimate, reticulocyte count, and the percentage of stomatocytes in blood films were microscopically evaluated. An osmotic fragility test of RBCs and measurement of intracellular Na+, K+, and 2,3-diphosphoglycerate (2,3-DPG) concentrations were also performed.

RESULTS

The affected dogs had macrocytosis (80.0 +/- 4.2 fL, reference interval 60-76 fL), decreased MCHC (29.3 +/- 0.8 g/dL, reference interval 32-39 g/dL), slightly increased RDW (17.3 +/- 0.4%, reference interval 12-16%), and an increased reticulocyte count (1.55 +/- 0.77%, reference interval <1%). The percentage of stomatocytes in blood films varied from 0.6 to 18.9% of all RBCs. Erythrocyte osmotic fragility and intracellular Na+ (138.1 +/- 3.2 mmol/L; controls 99 +/- 6.1 mmol/L), K+ (8.1 +/- 0.8 mmol/L; controls 6.1 +/- 0.5 mmol/L), and 2,3-DPG (21.9 +/- 2.0 micromol/g Hb; controls: 14.6 +/- 3.3 micromol/g Hb) concentrations were increased in dogs with stomatocytosis.

CONCLUSIONS

Hematologic findings and the metabolic defects in RBCs in these Standard Schnauzers were consistent with a diagnosis of stomatocytosis. Parentage analysis suggests that stomatocytosis in Standard Schnauzers may have a hereditary component.

摘要

背景

遗传性犬口腔红细胞增多症已在纯种阿拉斯加雪橇犬、荷兰猎鸟犬和迷你雪纳瑞犬中被描述。在人类中，遗传性口腔红细胞增多症是一组先天性疾病的异质性群体，其特征是血液中存在口腔红细胞、渗透脆性增加，且常伴有溶血性贫血。

目的

我们的目的是描述7只患有口腔红细胞增多症的亲缘关系密切的标准雪纳瑞犬的血液学检查结果和红细胞特征。

方法

使用自动分析仪测量以下参数：血细胞比容（HCT）、红细胞（RBC）、血红蛋白（Hb）浓度、平均红细胞体积（MCV）、平均红细胞血红蛋白含量（MCH）、平均红细胞血红蛋白浓度（MCHC）、红细胞分布宽度（RDW）、白细胞（WBC）、血小板计数、平均血小板体积（MPV）、血小板压积（PCT）和血小板分布宽度（PDW）。通过显微镜评估白细胞分类计数、血小板估计、网织红细胞计数以及血片中口腔红细胞的百分比。还进行了红细胞渗透脆性试验以及细胞内钠（Na+）、钾（K+）和2,3-二磷酸甘油酸（2,3-DPG）浓度的测量。

结果

患病犬出现大红细胞症（80.0±4.2 fL，参考区间60 - 76 fL）、MCHC降低（29.3±0.8 g/dL，参考区间32 - 39 g/dL）、RDW略有升高（17.3±0.4%，参考区间12 - 16%）以及网织红细胞计数增加（1.55±0.77%，参考区间<1%）。血片中口腔红细胞的百分比在所有红细胞中从0.6%到18.9%不等。患有口腔红细胞增多症的犬红细胞渗透脆性以及细胞内Na+（138.1±3.2 mmol/L；对照组99±6.1 mmol/L）、K+（8.1±0.8 mmol/L；对照组6.1±0.5 mmol/L）和2,3-DPG（21.9±2.0 μmol/g Hb；对照组：14.6±3.3 μmol/g Hb）浓度升高。