Bényei Balázs, Komlósi István, Pécsi Anna, Pollott Geoffry, Marcos Cruvinel Heraldo, de Oliveira Campos Alexandre, Lemes Maida Paula
Hungarian Academy of Sciences, Institute of Experimental Medicine, Szigony Street 43, Budapest 1083, Hungary.
Anim Reprod Sci. 2006 Jul;93(3-4):268-79. doi: 10.1016/j.anireprosci.2005.07.012. Epub 2005 Oct 5.
The objectives of this work were to determine the effect of external (synchronization methods, month, embryo origin and farm effects) and internal factors (age and size of CL, embryo development and quality score, synchronization methods, age of recipient, quality of transfer and reuse of recipients) on a commercial embryo transfer program in a tropical environment. In the program 1466 Holstein-Friesian purchased embryos were implanted to zebu/European crossbred recipients under field conditions. There were 502 pregnancies detected in this large-scale extension programme. Synchronization methods, month, embryo origin, and farm effects were found to have affected the success rate of embryo transfer. Due to the hot climate and large distances between recipient farms, seasonal effects, reused recipient pregnancy results and the effect of embryo development stage differed from previously reported results. Investigation by ultrasonograph showed that embryo loss occurred before 35 days of pregnancy. Under field conditions, routine fetal sexing resulted in <5% misidentification. In conclusion, under tropical conditions external factors have a major influence on the results of pregnancy from embryo transfer.
本研究的目的是确定外部因素（同步方法、月份、胚胎来源和养殖场效应）和内部因素（黄体的年龄和大小、胚胎发育和质量评分、同步方法、受体年龄、移植质量和受体的重复使用）对热带环境下商业胚胎移植项目的影响。在该项目中，1466枚购买的荷斯坦-弗里生胚胎在田间条件下移植到瘤牛/欧洲杂交受体母牛体内。在这个大规模推广项目中检测到502例妊娠。结果发现，同步方法、月份、胚胎来源和养殖场效应均对胚胎移植成功率产生了影响。由于气候炎热且受体养殖场之间距离较远，季节效应、受体重复使用的妊娠结果以及胚胎发育阶段的影响与先前报道的结果有所不同。超声检查发现，胚胎丢失发生在妊娠35天之前。在田间条件下，常规胎儿性别鉴定的错误识别率<5%。总之，在热带条件下，外部因素对胚胎移植妊娠结果有重大影响。
