Rao Yerra Koteswara, Fang Shih-Hua, Tzeng Yew-Min
Institute of Biotechnology, Chaoyang University of Technology, Wufeng 413, Taiwan, ROC.
J Ethnopharmacol. 2006 Jan 16;103(2):181-6. doi: 10.1016/j.jep.2005.07.021. Epub 2005 Oct 5.
Four compounds, including one benzenoid, 4-O-methylgallic acid (1), together with three arylnaphthalide lignans, namely phyllamyricin C (2), justicidin B (3) and diphyllin (4) were isolated from the whole plants of Phyllanthus polyphyllus L. (Euphorbiaceae). This was the first isolation report of compounds 1-4 from this plant species. The in vitro inhibitory effects of these compounds were evaluated on the production of nitric oxide (NO) and cytokines (tumor necrosis factor (TNF)-alpha and interleukin (IL)-12), from LPS/IFN-gamma activated murine peritoneal macrophages. The results indicated that the 50% inhibition concentration (IC(50)) values of NO production from activated peritoneal macrophages by compounds 1-4 were 100, 25, 12.5 and 50 microM, respectively. In parallel, these dilutions were approximately inhibited in a similar manner to that observed for cytokines (TNF-alpha, and IL-12) production. On the other hand, at 100 microM concentration compounds 3 and 4 showed 50% inhibition of NO production from peritoneal macrophages that had been pre-activated with LPS/IFN-gamma for 24h, whereas compounds 1 and 2 inhibited only about 20 and 10%, respectively. These results support the use of this plant for the treatment of inflammatory diseases in oriental traditional medicine.
从大戟科叶下珠全草中分离得到4种化合物，包括1种苯类化合物4-O-甲基没食子酸（1），以及3种芳基萘类木脂素，即叶下珠素C（2）、异叶下珠素B（3）和二叶豆素（4）。这是首次从该植物中分离得到化合物1-4的报道。评估了这些化合物对脂多糖/干扰素-γ激活的小鼠腹腔巨噬细胞产生一氧化氮（NO）和细胞因子（肿瘤坏死因子（TNF）-α和白细胞介素（IL）-12）的体外抑制作用。结果表明，化合物1-4对激活的腹腔巨噬细胞产生NO的50%抑制浓度（IC50）值分别为100、25、12.5和50 μM。同时，这些稀释度对细胞因子（TNF-α和IL-12）产生的抑制方式与NO产生的抑制方式相似。另一方面，在100 μM浓度下，化合物3和4对用脂多糖/干扰素-γ预激活24小时的腹腔巨噬细胞产生的NO有50%的抑制作用，而化合物1和2分别仅抑制约20%和10%。这些结果支持了该植物在东方传统医学中用于治疗炎症性疾病。
